D7295

Deoxynucleotide Mix, 10 mM

Molecular Biology Reagent

• Synonym(s): Deoxynucleotide Mix, 10 mM, 10mM dNTP mix, dNTP mix, dNTPs
• NACRES: NA.52
• UNSPSC Code: 41106305
• Assay: ≥99%
• Biological source: synthetic (organic)
• Form: liquid
• Solubility: H₂O: soluble at 20 °C
• Storage temp.: −20°C

Properties

• biological source: synthetic (organic)
• Quality Level: 300
• assay: ≥99%
• form: liquid
• concentration: 10 mM
• color: colorless
• solubility: H₂O: soluble at 20 °C
• application(s): agriculture
• foreign activity: DNase, RNase, none detected
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Nucleotides are organic molecules that serve as the monomers, or subunits, of nucleic acids (like DNA and RNA). The building blocks of nucleic acids, nucleotides consist of a nitrogenous base (purine or pyrimidine), a five-carbon sugar (ribose or deoxyribose), and at least one phosphate group. A nucleoside along with a phosphate group yields a nucleotide. The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM each of UltraPure dATP, dCTP, dGTP, and TTP sodium salts in high-quality molecular biology grade water. One µL is sufficient for a standard 50 µL PCR reaction.

Application

dNTP Mix has been used:

• in the PCR amplification of genomic DNA isolated from insect, fungi,[1] virus, human,[2]
• in reverse transcription of total RNA to cDNA.[3][3]
• as a component of the DNA amplification mixture for polymerase chain reaction (PCR)[1][4]
• routine and long PCR
• manual and automated DNA sequencing
• cDNA synthesis[5] and labeling reactions

Features and Benefits

• Purity of each dNTP: Minimum 99%
• Conveniently formulated; 1 μL is used per 50 μL PCR
• Equimolar amounts of each dNTP means less pipetting
• Minimize risk of contamination in PCR
• UltraPure dNTPs can help maximize consistency and yields in critical PCR reactions

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable