C7492

Monoclonal Anti-Calreticulin antibody produced in mouse

clone TO-11, tissue culture supernatant, buffered aqueous solution

• Synonym(s): Calreticulin Antibody Flow Cytometry, Anti-Autoantigen Ro
• NACRES: NA.44
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: TO-11, monoclonal
• Application: ELISA (i), FACS, ICC, IHC, WB
• Citations: 10

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: tissue culture supernatant
• antibody product type: primary antibodies
• clone: TO-11, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~50 kDa
• species reactivity: human
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, western blot: 1:50-1:100 using total cell extract of HeLa cells
• isotype: IgG1
• UniProt accession no.: P27797
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CALR(811)

Description

General description

Monoclonal Anti-Calreticulin (mouse IgG1 isotype) is derived from the hybridoma TO-11 produced by the fusion of mouse myeloma cells (P3-X63-AG8.653 cells) and splenocytes from BALB/c mice. Calreticulin is a molecular chaperones in endoplasmic reticulum lumen. Calreticulin acts as a lectin-like chaperone binding oligosaccharide residues of newly synthesized N-linked glycoproteins, and misfolded proteins. Calreticulin is a protein binding Ca²⁺ ions. It is encoded by the CALR gene in humans.

Application

Monoclonal Anti-Calreticulin antibody produced in mouse is suitable for the following applications:

• Flow cytometry
• Immunocytochemistry
• Immunohistochemistry
• Indirect ELISA
• Western blotting[1]

Biochem/physiol Actions

Calreticulin and Its variants causes differential effects on thrombopoiesis. Somatic mutations of calreticulin (CALR) gene causes thrombocythemia (ET) and primary myelofibrosis (PMF).

It is believed to play a critical role in quality control processes during protein synthesis and folding. Increased expression of calreticulin increases Ca²⁺ storage capacity of the ER. It also appears to modulate store-operated Ca²⁺ -influx, and to alter Ca²⁺ transport by the sarcoplasmic/ER Ca²⁺ -ATPase (SERCA). Overexpression of calreticulin results in increased sensitivity of HeLa cells to drug-induced apoptosis. In contrast, calreticulin-deficient cells show increased resistance to apoptosis. Calreticulin gene disruption leads toimpairment of cardiac development. Loss of calreticulin function favors ubiquitin-proteosome activity. It plays a critical role in Ca²⁺ homeostasis.

Physical form

Culture supernatant solution containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)