This product is not sterile, and after dissolving it will need to be sterile filtered with a 0.22 µm filter.
The lyophilized powder can be reconstituted in sterile 0.5 M acetic acid, water, or PBS to a concentration of 1 mg/mL.
A PBS solution is stable for at least 1 year at –20 °C.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/259/550/c5533pis.pdf
C5533
Human Collagen Type IV
from human placenta, powder, suitable for cell culture
Collagen from human placenta
Synonym(s):
Collagen Type 4
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| Pack Size | SKU | Availability | Price |
|---|
About This Item
Biological source:
human placenta
Form:
powder
Technique(s):
cell culture | mammalian: suitable
Pricing and availability is not currently available.
Product Name
Collagen from human placenta, Bornstein and Traub Type IV, powder, BioReagent, suitable for cell culture
biological source
human placenta
Quality Level
product line
BioReagent
form
powder
packaging
pkg of 5 mg
technique(s)
cell culture | mammalian: suitable
impurities
HIV, hepatitis B and hepatitis C, none detected
solubility
acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)
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This Item | |||
|---|---|---|---|
| biological source human placenta | biological source human placenta | biological source human placenta | biological source human placenta |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | stem cell: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form powder | form powder | form powder | form powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| binding specificity Peptide Source: Collagen Type IV | binding specificity - | binding specificity - | binding specificity - |
General description
All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type IV collagen occurs only in the basement membranes and contains up to six genetically distinct a-chains. Type IV collagens are localized to the basement membrane and they are network-forming proteins. It is encoded by six different genes: collagen type IV α1 (COL4A1), COL4A2, COL4A3, COL4A4, COL4A5 and COL4A6. Both COL4A1 and COL4A2 genes are mapped to human chromosome 13q34. The proteins encoded by the two genes share 45% homology. They contain: a non-collagenous (NC1) domain, triple-helical collagenous domain and Gly-Xaa-Yaa repeats in common. Both COL4A3 and COL4A4 genes are located on human chromosome 2q36.3. In tissues, type IV collagens are present as procollagens.
Application
Collagen from human placenta has been used:
- To solubilize extracellular matrix for surface functionalization.
- To induce endothelial cell [1] and rat pluripotent cell differentiation.
- As an adherent in cell migration/invasion assay.
- To assess the bioelectric effects of quinine on airway epithelial cells.
- To study the selective toxicity of engineered lentvirus lytic peptides.
- In particle aggregation assay for the rapid detection of fibronectin, fibrinogen and collagen receptors on Staphylococcus aureus.
- In a magnesium-dependent, collagen-binding assay during characterization of human lung tumor-associated antigens.
Biochem/physiol Actions
Type IV collagen maintains cell division and growth. Abnormal collagen secretion affects basement membrane formation. The collagen type IV, α1 (COL4A1) gene is overexpressed in gastric cancer. Mutation in COL4A1 contributes to intracerebral hemorrhage in adults and children. Cerebrovascular disease is resulted due to COL4A1 and COL4A2 mutations. Type IV collagen has been found to play a key role in angiogenesis, neurological diseases and metastasis.
During development, collagen IV is ubiquitously distributed in the basement membranes (BMs). During the maturation process, this network gets partially replaced in a remarkably tissue specific manner, defining the BM structure and function. Collagen IV has been shown to bind to platelets, hepatocytes, keratinocytes, endothelial, mesangial, pancreatic cells and some tumor cells.
Tissue injury in the autoimmune disease, Goodpasture syndrome, is due to the pathogenic autoantibodies targeting the collagen IV α3 chain. Mutations in the COL4A5 gene are associated with Alport syndrome.
During development, collagen IV is ubiquitously distributed in the basement membranes (BMs). During the maturation process, this network gets partially replaced in a remarkably tissue specific manner, defining the BM structure and function. Collagen IV has been shown to bind to platelets, hepatocytes, keratinocytes, endothelial, mesangial, pancreatic cells and some tumor cells.
Tissue injury in the autoimmune disease, Goodpasture syndrome, is due to the pathogenic autoantibodies targeting the collagen IV α3 chain. Mutations in the COL4A5 gene are associated with Alport syndrome.
Preparation Note
This is a lyophilized powder that can be reconstituted in sterile .5 M acetic acid, PBS or water at 1 mg/mL. A PBS solution will be stable for at least one year at -20°C. It was prepared by a modification of the pepsin extraction method of Niyibizi, C. et. al
Other Notes
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.
Disclaimer
This product should be stored desiccated at -20°C, and will retain activity in these conditions for 3 years.
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Find documentation for the products that you have recently purchased in the Document Library.
Genetic Variations of the COL4A1 Gene and Intracerebral Hemorrhage Risk: A Case-Control Study in a Chinese Han Population
Lin S, et al.
World Neurosurgery, 112, e527-e533 (2018)
Genotype-phenotype correlations in pathology caused by collagen type IV alpha 1 and 2 mutations
Jeanne M and Gould DB
Matrix Biology, 57, 29-44 (2017)
Color Atlas of Dermatoimmuno Histocytology, 62-62 (1989)
Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis
Banushi B, et al.
Nature Communications, 7, 12111-12111 (2016)
Phenotype variability in a large Spanish family with Alport syndrome associated with novel mutations in COL4A3 gene
Cervera AC, et al.
BMC Nephrology, 18(1), 325-325 (2017)
Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C5533-5MG | 04061833502259 |
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Is this material sterile or does it need to be filter-sterilized for cell culture?
1 answer-
Helpful?
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I want to know its molecular weight.
1 answer-
This product is a collagen Type IV, which contains up to 6 distinct a-chains. The molecular weight may be quite variable and is not determined on a lot-to-lot basis. Historical investigations reveal 3 bands on SDS-PAGE gels with corresponding molecular weights as follows:
Band 1: 102 - 104 kDa
Band 2: 157 - 162 kDa
Band 3: 178 - 181 kDaIn addition, the Merck Index reports a molecular weight of 130 kDa and the CRC reports a molecular weight of 95 kDa or greater.
(Merck Index, 11th ed., p. 387, #2476 / CRC-Collagen, vol. I, Biochemistry, 140 (1988))Helpful?
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Hi, I have several questions about collagen coating. 1.when we dissolve the collagenIV powder in 0.5M acetic acid, can it be stored at -30 degree? 2. When we dilute the collagen stock with 0.25% acetic acid, how long can i store it at 4 degree?
1 answer-
Solutions of 1 -2 mg/mL collagen in 0.5M acetic acid should be stable for at least 1 year at -20°C.
Collagen solutions stored at 2-8°C should remain stable only for a few days up to 1 week.Helpful?
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Goodmorning, I noticed that in the upper instructions it is stated to use acetic acid but below it seems to be also possible to use water/PBS. What holds true? And is the powder itself stable?
1 answer-
As mentioned on the product page under 'Preparation Note'' in the DESCRIPTION section, this lyophilized powder can be reconstituted in sterile 0.5 M acetic acid, water, or PBS to a concentration of 1 mg/mL. The product is tested for solubility in acetic acid at 1 mg/mL. The powder is not assigned an expiration date. It is assigned a retest schedule of every 3 years and is quite stable if stored properly.
Please see the link below to review the product data sheet for more information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/259/550/c5533pis.pdfHelpful?
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