C3865

Anti-N-Cadherin antibody, Mouse monoclonal

clone GC-4, purified from hybridoma cell culture

• Synonym(s): Anti-A-CAM, Anti-A-Cell Adhesion Molecule
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00164511
• Conjugate: unconjugated
• Clone: GC-4, monoclonal
• Application: EM, FACS, ICC, IHC, WB
• Citations: 110

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: GC-4, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 135 kDa
• species reactivity: chicken, monkey, mouse, human, rabbit, rat
• technique(s): electron microscopy: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: 10-20 μg/mL using rat cardiac muscle frozen sections, western blot: 10-20 μg/mL using chicken cardiac muscle or COS-7 cell extracts
• isotype: IgG1
• UniProt accession no.: P19022
• application(s): research pathology
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CDH2(1000) ; mouse ... Cdh2(12558) ; rat ... Cdh2(83501)

Description

General description

N-Cadherin is a member of the cadherin family of proteins that mediate cell-cell adhesion in a calcium dependent manner. N-cadherin is a transmembrane glycoprotein that localizes in the cell-cell adherens junction on the plasma membrane. The protein is known to be localized in the cell membrane.

Application

Anti-N-Cadherin antibody is suitable for the following procedures:

• electron microscopy
• flow cytometry
• immunocytochemistry[1]
• immunohistochemistry at a concentration of 10-20μg/mL using rat cardiac muscle frozen sections
• western blot at a concentration of 10-20μg/mL using chicken cardiac muscle or COS-7 cell extracts

Biochem/physiol Actions

CDH11 gene encodes a type II classical cadherin that mediate calcium-dependent cell-cell adhesion.

Higher expression of CDH2 gene was observed in tumor cells. It is known to induce the invasive and chemoresistant attributes to the cancer cells. N-glycosylation of N-cadherin is associated with the process of tumorigenesis especially in mammary gland, renal system, and ovary. Loss of expression of the gene was observed in adrenocortical carcinoma.

Physical form

0.2 μm-filtered solution in 0.01 M phosphate buffered saline, pH 7.4.

Other Notes

Clone GC-4 is also known as GB-9.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves