Product A9594, Monoclonal ANTI-FLAG® M2-Cy3™ antibody produced in mice, is mouse IgG1. Theoretically, it will be recognized by the anti-mouse IgG secondary antibody. However, Product A9594 is already conjugated with Cy3 so a secondary antibody is not needed for detection.
A9594
Monoclonal ANTI-FLAG® M2-Cy3 antibody, Mouse monoclonal
clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)
Synonym(s):
Monoclonal ANTI-FLAG® M2, Anti-ddddk, Anti-dykddddk
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|
About This Item
NACRES:
NA.32
UNSPSC Code:
41106514
Conjugate:
CY3 conjugate
Clone:
M2, monoclonal
Application:
IF (d)
Citations:
42
Pricing and availability is not currently available.
biological source
mouse
Quality Segment
conjugate
CY3 conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)
species reactivity
all
concentration
~1 mg/mL
technique(s)
direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
dry ice
storage temp.
−20°C
General description
Monoclonal ANTI-FLAG M2-Cy3 (mouse IgG) antibody is covalently conjugated to cyanine dye Cy3. The antibody conjugate binds to FLAG fusion proteins, and will recognize the FLAG sequence at the N-terminus, Met-N-terminus, or C-terminus of FLAG fusion proteins.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
For simple, one-step detection by immunocytochemistry. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies might cause cross-reactivity.
Learn more product details in our FLAG® application portal.
Learn more product details in our FLAG® application portal.
Physical form
Solution in phosphate buffered saline plus 1% BSA and preservative
Preparation Note
Dilute the antibody in Tris buffered saline (TBS): 0.05 M Tris,pH 7.4, with 0.15 M NaCl.
Other Notes
Suggested concentration of 1-10 mg/ml for immunocytochemistry.
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Cy3 is a trademark of Cytiva
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
1 of 1
This Item | |||
|---|---|---|---|
| conjugate CY3 conjugate | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form affinity isolated antibody |
| clone M2, monoclonal | clone M1, monoclonal | clone M5, monoclonal | clone M2, monoclonal |
| biological source mouse | biological source (Bioreactor) | biological source mouse | biological source mouse |
| species reactivity all | species reactivity all | species reactivity all | species reactivity all |
| form buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate) | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4) |
Still not finding the right product?
Try our Product Selector Tool to narrow your options
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A9594-.2MG | 04061833403624 |
| A9594-5X1MG | 04061833481530 |
| A9594-1MG | 04061826671092 |
-
A9594（FLAG M2 Cy3標識）の抗体は抗マウスIgGの2次抗体に認識されますか。
1 answer-
Helpful?
-