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A4058

Acrylamide solution

40%, suitable for electrophoresis, sterile-filtered

Synonym(s):

2-Propenamide, 40% Acrylamido solution, Acrylic amide, Akrylamid, Polyacrylamide solution

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About This Item

Linear Formula:
CH2=CHCONH2
CAS Number:
Molecular Weight:
71.08
NACRES:
NB.22
PubChem Substance ID:
eCl@ss:
39031205
UNSPSC Code:
41105319
MDL number:
Beilstein/REAXYS Number:
6185892
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sterility

sterile-filtered

Quality Segment

form

liquid

concentration

40%

technique(s)

electrophoresis: suitable

storage temp.

2-8°C

SMILES string

NC(=O)C=C

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

InChI key

HRPVXLWXLXDGHG-UHFFFAOYSA-N

General description

Acrylamide has been classified as probably carcinogenic in humans. It is formed in fried and oven-cooked foods due to Maillard reaction between amino acids and reducing sugars.[1]

Application

Acrylamide solution is suitable for use in electrophoresis. It may be used in the preparation of gel buffer for the determination of molecular weight of polypeptide chains by polyacrylamide gel electrophoresis.[2] It may be used in the electrochemical polymerization of pyrrole. It may be used to prepare the pH 7.6 gels, required for the determination of molecular weights of histone by sodium dodecyl sulfate gel electrophoresis.[3]

Preparation Note

Prepared from electrophoresis grade acrylamide in ultra-pure water.

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This Item
A8887A909901696
form

liquid

form

crystals

form

crystals

form

powder blend

concentration

40%

concentration

-

concentration

-

concentration

-

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

electrophoresis: suitable

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

sterility

sterile-filtered

sterility

-

sterility

-

sterility

-

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

2-8°C


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pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter



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Global Trade Item Number

SKUGTIN
A4058-100ML04061838254641
A4058-5L04061833368466

Questions

1–4 of 4 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the ratio of acrylamide:bisacrylamide in this product? Is it 37.5:1, 29:1 or 19:1 (or maybe other?)

    1 answer
    1. This product is simply a 40% solution of Acrylamide and does not contain any Bis-acrylamide. Note that there are several Acrylamide/Bis-acrylamide 40% solutions available. Please see the link below to review these product options:
      https://www.sigmaaldrich.com/substance/acrylamidebisacrylamide40solution1234598765

      Helpful?

  4. What is the solvent in this solution. Water? Or organic solvent?

    1 answer
    1. The solution is prepared from electrophoresis grade acrylamide in ultra-pure water and aseptically filtered to insure the highest quality and reproducibility.

      Helpful?

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