For batch release, this product’s appearance in water is tested at a concentration of 10 mg/mL. The observed color range is from faint green to green to green-yellow.
A1888
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt
≥98% (HPLC), chromogenic, powder
Synonym(s):
AzBTS-(NH4)2, Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
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About This Item
Empirical Formula (Hill Notation):
C18H24N6O6S4
CAS Number:
Molecular Weight:
548.68
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
250-396-6
MDL number:
Beilstein/REAXYS Number:
7329461
Pricing and availability is not currently available.
Product Name
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ≥98% (HPLC)
Quality Level
assay
≥98% (HPLC)
form
powder
solubility
water: 10 mg/mL, clear to slightly hazy
storage temp.
2-8°C
SMILES string
[NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O
InChI
1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;
InChI key
OHDRQQURAXLVGJ-AXMZSLBLSA-N
General description
ABTS, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), is water soluble chemical compound used as chromogenic substrate:
ABTS mainly used in ELISA (enzyme-linked immunosorbent assay) procedures.
A peroxidase reaction of ABTS in the presence of hydrogen peroxide produces a green soluble end product which can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS).††† ABTS, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive.
- ABTS together with hydrogen peroxide for reaction with peroxidase enzymes (like HRP for example).
- ABTS together with laccase or bilirubin oxidase.
ABTS mainly used in ELISA (enzyme-linked immunosorbent assay) procedures.
A peroxidase reaction of ABTS in the presence of hydrogen peroxide produces a green soluble end product which can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS).††† ABTS, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive.
Application
- 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt has been used as a substrate in ELISA (enzyme-linked immunosorbent assay).
- ABTS is significantly used by the food industry, to measure the antioxidant capacities within different foods.
- ABTS is also used to monitor glucose concentrations in different solutions including blood serum.
- It is recommended for ELISA (microwell) procedures, not recommended for membrane applications.Since it is less readily oxidized than TMB and OPD substrates, ABTS is less sensitive for ELISA applications. Thus ABTS has some advantageous over TMB and OPD in cases of large background results.
2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
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This Item | |||
|---|---|---|---|
| assay ≥98% (HPLC) | assay - | assay ≥98% (HPLC) | assay ≥98% (HPLC) |
| solubility water: 10 mg/mL, clear to slightly hazy | solubility deionized water: 100 mL | solubility H2O: 10 mg/mL, slightly hazy to clear | solubility - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| form powder | form tablet | form powder or crystals | form crystals, powder |
| storage temp. 2-8°C | storage temp. room temp | storage temp. 2-8°C | storage temp. 2-8°C |
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Storage Class
11 - Combustible Solids
wgk
WGK 2
ppe
dust mask type N95 (US), Eyeshields, Gloves
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Product Information Sheet
Expression of glucocorticoid resistance following social stress requires a second signal.
Avitsur R et al.
Journal of Leukocyte Biology, 74, 507-507 (2003)
Structure-based mutagenesis of herpes simplex virus glycoprotein D defines three critical regions at the gD-HveA/HVEM binding interface.
Connolly SA et al.
Journal of Virology, 77, 8127-8127 (2003)
Evaluation of ELISA with ABTS, 2-2'-azino-di-(3-ethylbenzthiazoline sulfonic acid), as the substrate of peroxidase and its application to the diagnosis of schistosomiasis.
Matsuda H et al.
The Japanese Journal of Experimental Medicine, 54, 131-131 (1984)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A1888-5G | 04061835504374 |
| A1888-2G | 04061835551224 |
| A1888-1G | 04061835551217 |
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What is the color of ABTS solution after dissolving ABTS in water?
1 answer-
Helpful?
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What is the concentration of Product A1888, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt?
1 answer-
This product is available in powder form and is not a prepared solution. Should a prepared solution be preferred, please see the link below:
https://www.sigmaaldrich.com/US/en/product/roche/11684302001Helpful?
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What is the weight of 1G?
1 answer-
This product is available in 1, 2, and 5 gram quantities. The G in 1G represents 'gram'. The total packing weights, including the bottle, are 6 grams, 8 grams, and 18 grams, respectively.
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