This product is soluble at 1 mM in water. The Certificate of Analysis recommends using sterile distilled water. Tissue culture grade water is suitable.
SCM075
ROCK Inhibitor (Y-27632)
The ROCK Inhibitor (Y-27632) is available in a 5 mg format & has been optimized & validated for cell culture & Neuroscience applications.
Synonym(s):
Y-27632
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|
About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
eCl@ss:
32160801
Pricing and availability is not currently available.
Quality Segment
form
liquid
mol wt
320.26 g/mol
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
-10 to -25°C
General description
ROCK Inhibitor (Y-27632) is a selective, ATP-competitive inhibitor of Rho-associated protein kinase (ROCK) including p160Rock (Ki=140nM) and Rock-II. Also acts as a potent inhibitor of agonist-induced Ca2+ sensitization of myosin phosphorylation and smooth muscle contraction. One-hour treatment with 10uM Y-27632 blocks apoptosis of dissociated cultured human embryonic stem cells (hESCs), increases survival and cloning efficiency of hESCs without affecting their pluripotency1. Recently, some reports showed that the application of Y-27632 and Accutase (Cat. No. SCR005), a formulated mixture of digestive enzymes, enhances the post-thaw survival rate of single hESCs using conventional slow freezing and rapid thawing methods
Preparation Note
Before Reconstitution: Maintain at -20ºC, protected from light for 2 years. After Reconstitution: 1 year at -20ºC, protected from light
1 of 1
This Item | |||
|---|---|---|---|
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| form liquid | form solid | form liquid | form liquid |
| storage temp. -10 to -25°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in ambient | shipped in wet ice | shipped in - |
| mol wt 320.26 g/mol | mol wt - | mol wt - | mol wt - |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) - |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| SCM075 | 04053252374654 |
-
How to dissolve RI? Can I use cell culture grade water?
1 answer-
Helpful?
-