AB3468

Anti-Heat Shock Protein 90β Antibody

Chemicon^®, from rabbit

• Synonym(s): HSP84
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ICC, IHC, IP, WB
• Citations: 9

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, rat, mouse
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NM_007355.2
• UniProt accession no.: P08238
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... HSP90AA1(3320) ; mouse ... Hsp90Aa1(15519) ; rat ... Hsp90Aa1(299331)

Description

Immunogen

Synthetic peptide corresponding to amino acids 2-13 from the N-terminus of mouse heat shock protein 90b.

Application

Immunoblotting: 5 μg/mL

Immunohistochemistry (frozen and formalin/paraffin): 5-10 μg/mL. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

Immunocytochemistry

Immunoprecpitation: 10 μg/mg of protein lysate.

Optimal working dilutions must be determined by end user.

Research Category
Protein Trafficking

Research Sub Category
Chaperones

This Anti-Heat Shock Protein 90β Antibody is validated for use in IP, WB, IC, IH(P) for the detection of Heat Shock Protein 90β.

Biochem/physiol Actions

Reacts with a protein of 84 kD identified as heat shock protein 90beta (HSP90beta). No reactivity with HSP90alpha/HSP86.

CELLULAR LOCALIZATION: Nuclear and Cytoplasmic.

Physical form

Format: Purified

Purified immunoglobulin. Liquid in 10 mM PBS, pH 7.4 with 0.2% BSA and 15 mM sodium azide.

Preparation Note

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Analysis Note

Control
POSITIVE CONTROL: MAD109 cells or breast carcinoma.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2