AB3230

Anti-Low Density Lipoprotein Antibody, copper oxidized

serum, Chemicon^®

• Synonym(s): LDL
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: human
• Application: ELISA, IHC, WB
• Citations: 10

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, immunohistochemistry: suitable, western blot: suitable
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... LDLR(3949)

Description

Immunogen

Epitope: copper oxidized

Fully Cu²⁺-oxidized LDL.

Application

Anti-Low Density Lipoprotein Antibody, copper oxidized is an antibody against Low Density Lipoprotein for use in ELISA, WB, IH.

Research Category
Metabolism

Research Sub Category
Lipid Metabolism & Weight Regulation

Western Blot

Immunohistochemistry: 1:50-1:500

ELISA: 1:500-1:5,000

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Reacts strongly to fully oxidatized modifications of LDL including Cu2+-oxidized LDL, MDA-LDL, HOCL-LDL but not to other oxidized proteins like MDA-HSA, HOCL-HSA, MDA-HDL, HOCL-HDL (below detection limit). The reaction of native LDL was weak, but clearly detectable (approx. 20%). Minimally oxidized LDL gave a strong binding signal (>80%).

Physical form

Rabbit serum. Lyophilized. Contains no preservative. Reconstitute with 100 μL of sterile distilled water.

Preparation Note

Maintain lyophilized material at 2-8°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable