52332

Phenylmethylsulfonyl Fluoride

Phenylmethylsulfonyl Fluoride, CAS 329-98-6, is an irreversible inhibitor of serine proteases. It causes sulfonylation of the active-site serine residues.

• Synonym(s): Phenylmethylsulfonyl Fluoride, Benzylsulfonyl Fluoride, PMSF
• Empirical Formula (Hill Notation): C₇H₇FO₂S
• CAS Number: 329-98-6
• Molecular Weight: 174.19
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00007424
• Assay: ≥99% (GC)
• Form: crystalline solid
• Quality level: 200
• Storage condition: OK to freeze, desiccated

Properties

• Quality Level: 200
• description: RTECS - XT8040000
• assay: ≥99% (GC)
• form: crystalline solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated
• color: white to off-white
• solubility: ethanol: soluble, isopropanol: soluble, methanol: soluble
• shipped in: ambient
• storage temp.: 10-30°C
• SMILES string: F[S](=O)(=O)Cc1ccccc1
• InChI: 1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2
• InChI key: YBYRMVIVWMBXKQ-UHFFFAOYSA-N

Description

General description

Irreversible inhibitor of serine proteases. Its mechanism of action is analogous to that of diisopropylfluorophosphate. PMSF causes sulfonylation of the active-site serine residues. Also reported to inhibit internucleosomal DNA fragmentation in immature thymocytes. For a related, more stable inhibitor, see AEBSF (Cat. No. 101500).

Biochem/physiol Actions

Cell permeable: no

Primary Target
serine proteases

Product does not compete with ATP.

Reversible: no

Preparation Note

Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 9 months at 4°C.

t_½ = 1 hour at pH 7.5. Effective concentration: 50 μM.

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Seitz, R., et al. 1993. Int. J. Cancer53, 514.
Weaver, V.M., et al. 1993. Biochem. Cell. Biol.71, 488.
Bourgain, R.H., et al. 1992. Adv. Exp. Med. Biol.316, 427.
Chang, C.T., et al. 1992. Biochem. Int.28, 707.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Corrosive (L)

Safety Information

• pictograms: GHS06,GHS05
• signalword: Danger
• hcodes: H301,H314
• pcodes: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Hazard Classifications: Acute Tox. 3 Oral - Skin Corr. 1B
• Storage Class: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable