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SEQXE

Sigma-Aldrich

SeqPlex DNA Amplification Kit

For use with high throughput sequencing technologies, Whole Genome Amplification kit designed to facilitate Next Gen Sequencing.

Synonym(s):

SeqPlex Enhanced DNA Amplification Kit, WGA kit, whole genome amplification kit

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About This Item

UNSPSC Code:
41121800
NACRES:
NA.55

Quality Level

200

technique(s)

whole genome amplification: suitable

shipped in

wet ice

storage temp.

−20°C

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General description

SeqPlex™ Enhanced DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA. The yields from chromatin immunoprecipitation (ChIP) or formalin-fixed paraffin-embedded tissue samples (FFPE) are often less than required for successful next generation sequencing library preparation. The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented DNA samples for input into a NGS workflow. This kit is an extension of the WGA product line and has been developed to integrate into the Illumina®, SOLiD™, or 454 sequencing workflows.

Application

SeqPlex™ DNA Amplification Kit has been used for whole genome amplification.

Features and Benefits

  • Random priming technology amplifies fragmented DNA such as ChIP or FFPE
  • Facilitates sequencing from as little as 100 pg of ChIP DNA
  • Enhanced primers for complete genome coverage, minimal sequence bias, primer removal, and amplicon size ideal for next gen sequencing
  • Compatible with Illumina®, SOLiD™, or 454 library prep for next generation sequencing

Other Notes

SEQXE-500RXN is manufactured on-demand. Contact technical services at techserv@sial.com for more information.

Legal Information

Illumina is a registered trademark of Illumina, Inc.
SOLiD is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
iCAT is a registered trademark of University of Washington

Kit Components Also Available Separately

Product No.
Description
SDS
  • W4502Water, Nuclease-Free Water, for Molecular BiologySDS
  • Library Preparation Buffer

recommended

Product No.
Description
Pricing

required but not provided

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Optimizing sparse sequencing of single cells for highly multiplex copy number profiling.
Baslan T, et al.
Genome Research, 25(5), 714-724 (2015)
SF3B1 mutant MDS-initiating cells may arise from the haematopoietic stem cell compartment.
Mian S A, et al.
Nature Communications, 6, 10004-10004 (2015)
Dimiter Kunnev et al.
Journal of biological methods, 2(4) (2015-01-01)
Nascent strand capture and release (NSCR) is a method for isolation of short nascent strands to identify origins of DNA replication. The protocol provided involves isolation of total DNA, denaturation, size fractionation on a sucrose gradient, 5'-biotinylation of the appropriate
Timour Baslan et al.
Genome research, 25(5), 714-724 (2015-04-11)
Genome-wide analysis at the level of single cells has recently emerged as a powerful tool to dissect genome heterogeneity in cancer, neurobiology, and development. To be truly transformative, single-cell approaches must affordably accommodate large numbers of single cells. This is
Dimiter Kunnev et al.
Genome research, 25(4), 558-569 (2015-03-13)
Minichromosome maintenance (MCM) proteins are loaded onto chromatin during G1-phase and define potential locations of DNA replication initiation. MCM protein deficiency results in genome instability and high rates of cancer in mouse models. Here we develop a method of nascent
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Protocols

SeqPlex DNA Amplification Kit Protocol

SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.

SeqPlex DNA Amplification Kit Protocol

SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.

SeqPlex DNA Amplification Kit Protocol

SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.

SeqPlex DNA Amplification Kit Protocol

SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.

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