Really good value for money and always arrives with a good shelf-life in terms of expiry date. Great for adding cytokines + hydrocortisone to to create your own endothelial cell growth medium - has kept my HUVECs consistently happy since I started using it in 2018. Also great to use when isolating human primary endothelial cells.
M4530
Medium 199
With Earle′s salts, ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Medium 199
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(s):
M199 Medium, TCM 199
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Pricing and availability is not currently available.
Product Name
Medium 199, With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: yes
L-glutamine: yes
HEPES: no
Earle’s salts (5% CO2): yes
phenol red: yes
sodium pyruvate: no
shipped in
ambient
storage temp.
2-8°C
General description
Complete liquid version of the original M 199 formulation published by Morgan.
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Application
Medium 199 has been used:
- as a suspension media to maintain the primary neuronal cultures
- as a component of the collagen gel for ex vivo embryonic heart culture
- to maintain the ovarian tissue sections as part of the slow-freezing protocol
1 of 1
This Item | |||
|---|---|---|---|
| sterility sterile-filtered | sterility sterile-filtered | sterility - | sterility - |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form liquid | form liquid | form powder | form powder |
| components NaHCO3: yes | components sodium pyruvate: no | components L-glutamine: yes | components L-glutamine: no |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Li Ma et al.
The journals of gerontology. Series A, Biological sciences and medical sciences, 72(4), 489-498 (2016-08-28)
We previously reported that pretreatment with the potent antioxidant TEMPOL improves mitochondrial function and restores preconditioning in the aging heart. Because mitophagy is implicated in cardiac preconditioning and declines with age, this study was designed to investigate how age influences
Patrick C Baer et al.
International journal of molecular sciences, 19(6) (2018-06-13)
Stem cell-based therapies require cells with a maximum regenerative capacity in order to support regeneration after tissue injury and organ failure. Optimization of this regenerative potential of mesenchymal stromal/stem cells (MSC) or their conditioned medium by in vitro preconditioning regimens
Vladimir Jovasevic et al.
Journal of virology, 82(7), 3311-3319 (2008-01-25)
In this report we propose a model in which after the herpes simplex virus (HSV) capsid docks at the nuclear pore, the tegument protein attached to the capsid must be cleaved by a serine or a cysteine protease in order
Global Trade Item Number
| SKU | GTIN |
|---|---|
| M4530-100ML | 04061837847363 |
| M4530-6X500ML | 04061834059042 |
| M4530-1L | 04061834059028 |
| M4530-6X1L | 04061834059035 |
| M4530-500ML | 04061837847370 |