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386A

Leukocyte Acid Phosphatase (TRAP) Kit

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About This Item

NACRES:
NA.47
UNSPSC Code:
12352106
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Quality Level

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

dilution

(for histology)

application(s)

hematology
histology

shipped in

wet ice

storage temp.

2-8°C

Biochem/physiol Actions

Peripheral blood or bone marrow preparations are fixed to a microscope slide. The resulting film is incubated in a solution of Naphthol AS-BI phosphoric acid and Fast Garnet GBC. Kit is used to demonstrate acid phosphatase and tartrate resistant acid phosphatase (TRAP) in blood, bone marrow and tissue touch preparations.


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Kit Components Only

Product No.
Description

  • Acetate Solution (386-3) 50 mL

  • Acid Hematoxylin (285-2) 100 mL

  • Citrate Concentrate (386-1) 20 mL

  • Fast Garnet GBC Salt (kit only) 2 × 10

  • Naphthol AS-BI Phosphoric Acid (386-4) 40 mL

  • Tartrate Solution (kit only) 20 mL

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Repr. 1B - Skin Corr. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3



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Gui-Hua Quan et al.
International journal of molecular sciences, 16(12), 29496-29507 (2015-12-23)
Calycosin, an isoflavonoid phytoestrogen, isolated from Radix Astragali, was reported to possess anti-tumor, anti-inflammation, and osteogenic properties, but its impact on osteoclast differentiation remains unclear. In this study, we examined the effects of calycosin on osteoclastogenesis induced by RANKL. The
Annina Sipola et al.
BMC musculoskeletal disorders, 7, 56-56 (2006-07-15)
Endostatin is a C-terminal fragment of collagen XVIII which is a component of basement membranes with the structural properties of both collagens and proteoglycans. Endostatin has a major role in angiogenesis which is intimately associated with bone development and remodeling.
Xiao-Li Zhao et al.
Biochemical and biophysical research communications, 488(1), 15-21 (2017-05-04)
Aesculin (AES), a coumarin compound derived from Aesculus hippocasanum L, is reported to exert protective role against inflammatory diseases, gastric disease and cancer. However, direct effect of AES in bone metabolism is deficient. In this study, we examined the effects



Global Trade Item Number

SKUGTIN
386A-1KT04061831967241

Questions

1–2 of 2 Questions  
  1. Does the current version of product 386A-1KT include sodium nitrate?

    1 answer
    1. The 386A kit does not contain sodium nitrite, while the 387A kit includes sodium nitrite as part of the kit.

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  2. Is product 3873 equivalent to the tartrate solution used in the Leukocyte Acid Phosphatase (TRAP) kit (386A)?

    1 answer
    1. For the tartrate solutions in the 2 TRAP kits, 386A and 387A, if the 386A kit is not available, considering purchasing the 387A kit is recommended. The results are the same, although the kit composition varies from kit to kit. The Tartrate Solution in the 386A kit is 0.67M with a pH of 5.2, whereas the molarity of the Tartrate solution in the 387A kit is 0.67M and has a pH of 4.9. The instructions call for using 2 mL of the tartrate solution in Beaker B for the 386A procedure, and 1 mL of tartrate solution in Beaker B for the 387A procedure. There have been no studies showing whether the 2 reagents are interchangeable. It is best to use the 387A kit if the kit is available.

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