XNABE

REDExtract-N-Amp^™ Blood PCR Kit

sufficient for 100 extractions, sufficient for 500 amplifications

• NACRES: NA.55
• UNSPSC Code: 41106303

Properties

• Quality Level: 200
• usage: sufficient for 100 extractions, sufficient for 500 amplifications, sufficient for 500 reactions
• feature: dNTPs included, hotstart, Direct PCR
• technique(s): PCR: suitable
• color: red
• input: crude DNA
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

The REDExtract-N-Amp Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by PCR. This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration, or alcohol precipitation. The kit also includes a PCR ReadyMix^™, that uses an antibody based hot start and is specially formulated for amplification directly from the extract. The REDExtract-N-Amp Blood PCR Kit contains a tracking dye that allows for convenient direct loading of PCR reactions onto agarose gels for analysis.

Genomic DNA is extracted from 10 μL of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR Mix.

The REDExtract-N-Amp^™ Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by direct PCR (polymerase chain reaction). This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix^™, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification. The REDExtract-N-Amp Blood PCR Kit contains a tracking dye that allows for convenient direct loading of PCR reactions onto agarose gels for analysis.

Application

REDExtract-N-Amp^™ Blood PCR Kit has been used to extract genomic DNA from blood samples, peripheral blood leukocytes, parasites and endocervical swab eluates of mice.[1][2][3][4][5][6] It has also been used to perform polymerase chain reaction (PCR) using oligonucleotide primers.[7]

Biochem/physiol Actions

Genomic DNA is extracted from 10 μl of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR ReadyMix.

Features and Benefits

• Efficient 8 minute prep allows greater speed and throughput
• No need for organic extraction, centrifugation or alcohol precipitation
• Hot start antibody for highly specific PCR amplification of genomic DNA
• Can be used with whole blood or blood cards
• Extract stable at 4 °C for at least 6 months
• Compatible with any format (single tube, 96-well, etc.)
• Simple, 3 step procedure with no special equipment required
• REDExtract-N-Amp^™ requires no loading buffers or tracking dyes required for gel analysis
• PCR products are suitable for direct cloning and sequencing

Other Notes

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

• L3289

  Lysis Solution for Blood

  SDS
• N9784

  Neutralization Solution for Blood

  SDS
• P8240

  REDExtract-N-Amp^™ PCR ReadyMix^™ for Blood

  SDS

Safety Information

• pictograms: GHS05
• signalword: Danger
• hcodes: H290,H314
• pcodes: P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Hazard Classifications: Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
• Storage Class: 8A - Combustible corrosive hazardous materials