The composition of this aqueous Hemin solution is considered proprietary. This preparation does not include preservatives or organic solvents; however, buffer salts are present.
MBD0052
Hemin solution
Ready-made microbial growth medium supplement, 0.5 g/L
Synonym(s):
Chlorohemin solution, ChloroprotoporphyrinIX iron(III) solution, Ferriprotoporphyrin IX chloride Solution
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About This Item
NACRES:
NA.21
UNSPSC Code:
41106212
Pricing and availability is not currently available.
Quality Level
form
liquid
usage
supplement for growth media
concentration
0.5 g/L
shipped in
wet ice
storage temp.
2-8°C
General description
Hemin is a pro-oxidant, hemolysis derived-product.[1] It is essential for the growth of heme-deficient organisms such as trypanosomatids.[2] Hemin readymade solution is used as a culture-medium supplement for fastidious anaerobic microorganisms, shown to enhance the growth of many anaerobes including Bacteroides species and certain gram-positive non-spore forming organisms.[3] It is present as a key ingredient compound in common anaerobic growth media, such as Brucella broth, Thioglycolate broth and Reinforced Clostridial Medium.
Application
Hemin solution may be used as a supplement in brain heart infusion-yeast extract-hemin (BHI-YH) medium to determine cholesterol-to-coprostanol reducing activity.[4]
Features and Benefits
- Convenient readymade solution to speed up media preparation
- Sterile solution
- Optimal mixture for growth of fastidious bacteria as well as diversified culturing of microbial samples
Other Notes
- For 100 ml of medium preparation, it is recommended to add 1 mL hemin ready-made solution after autoclaving the medium, to achieve a final concentration of 5 microgram/ml. The optimal concentration of the supplement may vary for each strain.
- The solution is classified as light sensitive. 2. The solution should not be heated and must be added aseptically to autoclaved media cooled to 50-55°C. 3.
- Select strains which show turbid growth (24-48 hr.) with Hemin supplementation: Porphyromonas gingivalis, Bifidobacterium longum, Clostridium perfringens, Bacteroides vulgatus and Fusobacterium nucleatum.
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This Item | |||
|---|---|---|---|
| form liquid | form powder | form - | form powder or crystals |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in wet ice | shipped in - | shipped in ambient | shipped in - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| usage supplement for growth media | usage - | usage - | usage - |
| concentration 0.5 g/L | concentration - | concentration -100 × | concentration - |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
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