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Calcium chloride dihydrate

Name: Calcium chloride dihydrate
Brand: SIGMA

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%

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About This Item

Empirical Formula (Hill Notation):

CaCl₂ · 2H₂O

CAS Number:

10035-04-8

Molecular Weight:

147.01

NACRES:

NA.71

PubChem Substance ID:

24892994

UNSPSC Code:

12352302

EC Number:

233-140-8

MDL number:

MFCD00149613

Assay:

≥99.0%

Form:

powder

Solubility:

H₂O: 294 mg/mL, clear, colorless

Pricing and availability is not currently available.

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Properties

product line

BioReagent

Quality Level

200

assay

≥99.0%

form

powder

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable

mp

176 °C (dec.) (lit.)

solubility

H₂O: 294 mg/mL, clear, colorless

SMILES string

O.O.Cl[Ca]Cl

InChI

1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2

InChI key

LLSDKQJKOVVTOJ-UHFFFAOYSA-L

Description

General description

Calcium chloride (CaCl₂) is a commonly used reagent in biochemistry. It plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability. CaCl₂is used in the preparation and transformation of competent Escherichia coli (E. coli) and the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl₂-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. CaCl₂is used in the stabilization and two-dimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from E. coli.[1] The crystallization of porcine pancreatic elastase in the presence of CaCl₂along with sodium citrate reveals the binding of calcium in the metal-binding site of the protein.[2] The effect of CaCl₂on the total fluorescence in the polymerization of the tubulin-like filamenting temperature-sensitive mutant Z (FtsZ) division protein of E. coli has been studied by a multiwell fluorescent assay.[3]

Application

Calcium chloride dihydrate has been used:

in the formulation of media
in sperm collection and preparation
in cell culture and protoplast preparation

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This Item	12022	21097	C3306
Sigma-Aldrich C7902 Calcium chloride dihydrate	Sigma-Aldrich 12022 Calcium chloride dihydrate	Sigma-Aldrich 21097 Calcium chloride dihydrate	Sigma-Aldrich C3306 Calcium chloride dihydrate
form powder	form powder or crystals	form solid	form powder
solubility H₂O: 294 mg/mL, clear, colorless	solubility -	solubility H₂O: 1 M at 20 °C, clear, colorless	solubility H₂O: 294 mg/mL, clear, colorless
assay ≥99.0%	assay 99-103%	assay ≥99.5% (KT)	assay ≥99.0%
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
mp 176 °C (dec.) (lit.)	mp 176 °C (dec.) (lit.)	mp 176 °C (dec.) (lit.)	mp 176 °C (dec.) (lit.)
technique(s) cell culture \| insect: suitable, cell culture \| plant: suitable, cell culture \| mammalian: suitable	technique(s) -	technique(s) -	technique(s) -

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pictograms

GHS07

signalword

Warning

hcodes

H319

pcodes

P264 - P280 - P305 + P351 + P338 - P337 + P313

Hazard Classifications

Eye Irrit. 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Storage Class

11 - Combustible Solids

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