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Bovine Serum Albumin

Name: Bovine Serum Albumin
Brand: SIGMA

lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)

Synonym(s):

Albumin bovine serum, BSA, Bovine albumin

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About This Item

CAS Number:

9048-46-8

UNSPSC Code:

12352202

EC Number:

232-936-2

NACRES:

NA.27

MDL number:

MFCD00130384

eCl@ss:

32160409

Biological source:

bovine

Assay:

≥99% (agarose gel electrophoresis)

Form:

lyophilized powder

Technique(s):

blocking: suitable, microbiological culture: suitable

Impurities:

essentially globulin free

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Properties

biological source

bovine

Quality Level

300

assay

≥99% (agarose gel electrophoresis)

form

lyophilized powder

purified by

cold ethanol and heatshock fractionation

packaging

poly bottle of

origin

USA origin

technique(s)

blocking: suitable, microbiological culture: suitable

impurities

essentially globulin free

pH

solubility

water: soluble (50 mg/ml)

UniProt accession no.

P02769

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

Description

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Purified by a combination of heat shock and ethanol fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

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This Item	A3983	A0281	A3608
Sigma-Aldrich A7638 Bovine Serum Albumin	Sigma-Aldrich A3983 Bovine Serum Albumin	Sigma-Aldrich A0281 Bovine Serum Albumin	Sigma-Aldrich A3608 Bovine Serum Albumin
technique(s) blocking: suitable, microbiological culture: suitable	technique(s) western blot: suitable	technique(s) enzyme immunoassay: suitable	technique(s) immunoprecipitation (IP): suitable
origin USA origin	origin New Zealand origin	origin USA origin	origin New Zealand origin
assay ≥99% (agarose gel electrophoresis)	assay ≥98% (agarose gel electrophoresis)	assay ≥99% (agarose gel electrophoresis)	assay ≥96% (agarose gel electrophoresis)
biological source bovine	biological source bovine	biological source bovine	biological source bovine
Quality Level 300	Quality Level 200	Quality Level 300	Quality Level 200
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder

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Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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