Amberlite™ XAD 2 may be used for isolating flavonoids from plant extracts, as it is a hydrophobic resin commonly utilized for polyphenolic purification. This product is available in several package sizes, including a 100 G package as product 20275. Please see the link below to review all package sizes available, as well as a publication regarding the isolation of flavonoids:
https://www.sigmaaldrich.com/tech-docs/paper/432232
10357
Amberlite™ XAD®-2
pkg of 500 g
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About This Item
Pricing and availability is not currently available.
Quality Segment
agency
suitable for ASTM® D4861, suitable for EPA Method 10A, Method 23 and OTM-45
form
beads (small)
packaging
pkg of 500 g
technique(s)
HPLC: suitable, LPLC: suitable
surface area
~300 m2/g
matrix
styrene-divinylbenzene (macroreticular)
matrix active group
polymer
particle size
20-60 mesh
pore size
~0.65 mL/g pore volume, 90 Å mean pore size
density
1.02 g/mL at 25 °C (true wet)(lit.), 1.08 g/mL at 25 °C (skeletal)(lit.)
separation technique
reversed phase
storage temp.
2-8°C
SMILES string
N([C@@H](CCC(=O)OC(C)(C)C)C=O)C(=O)OCC1c2c(cccc2)c3c1cccc3
InChI
1S/C24H27NO5/c1-24(2,3)30-22(27)13-12-16(14-26)25-23(28)29-15-21-19-10-6-4-8-17(19)18-9-5-7-11-20(18)21/h4-11,14,16,21H,12-13,15H2,1-3H3,(H,25,28)/t16-/m0/s1
InChI key
BCIPGSZQUDLGSY-INIZCTEOSA-N
General description
Amberlite XAD-2, a hydrophobic copolymer of styrene-divinylbenzene resin, requires longer purification process and is used as a sorbent. Amberlite XAD-2 resin depending upon the aromaticity and size of the hydrophobic groups sorbs organic compounds through low-energy physical interactions.
Nonionic macroreticular resin that adsorbs and releases ionic species through hydrophobic and polar interactions; usually used under isocratic conditions.
Specifications:
Specifications:
Application
Amberlite™ XAD®-2 was used:
Polyaromatic adsorbent resin for hydrophobic compounds up to MW 20,000: phenols, organic removal, surfactants, aroma compounds, antibiotic recovery. May be used as support for catalyst or metals removal. For cleaned US EPA versions, see Supelpak-2 and Supelpak-2B.
Legal Information
ASTM is a registered trademark of American Society for Testing and Materials
Amberlite is a trademark of DuPont de Nemours, Inc.
XAD is a registered trademark of The Dow Chemical Company or an affiliated company of Dow
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This Item | |||
|---|---|---|---|
| particle size 20-60 mesh | particle size 20-60 mesh | particle size 20-60 mesh | particle size 20-60 mesh |
| matrix active group polymer | matrix active group polymer | matrix active group polymer | matrix active group polymer |
| separation technique reversed phase | separation technique reversed phase | separation technique reversed phase | separation technique reversed phase |
| pore size ~0.65 mL/g pore volume, 90 Å mean pore size | pore size ~0.65 mL/g pore volume, 90 Å mean pore size | pore size ~0.65 mL/g pore volume, 90 Å mean pore size | pore size ~0.65 mL/g pore volume, 90 Å mean pore size |
| matrix styrene-divinylbenzene (macroreticular) | matrix styrene-divinylbenzene (macroreticular) | matrix styrene-divinylbenzene (macroreticular) | matrix styrene-divinylbenzene (macroreticular) |
| technique(s) HPLC: suitable, LPLC: suitable | technique(s) HPLC: suitable, LPLC: suitable | technique(s) LPLC: suitable, solid phase extraction (SPE): suitable | technique(s) HPLC: suitable, LPLC: suitable |
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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M Tamura et al.
The Journal of biological chemistry, 260(17), 9672-9677 (1985-08-15)
Na+,K+-ATPase inhibitors have been found to exist in acutely saline-infused hog plasma, which also inhibit the specific binding of ouabain to Na+,K+-ATPase and the binding of digoxin to specific anti-digoxin antibody. Two of these inhibitors were purified by a combination
Trapping efficiency of polyurethane foam and Amberlite XAD-2 for various organochlorine compounds
Nerin, C., et al.
Fresenius Journal of Analytical Chemistry, 352, 609-611 (1995)
I Martos et al.
Journal of agricultural and food chemistry, 48(5), 1498-1502 (2000-05-23)
European Eucalyptus honeys showed a common and characteristic HPLC profile in which the flavonoids myricetin (3,5,7,3',4', 5'-hexahydroxyflavone), tricetin (5,7,3',4',5'-pentahydroxyflavone), quercetin (3,5,7,3',4'-pentahydroxyflavone), luteolin (5,7,3', 4'-tetrahydroxyflavone), and kaempferol (3,5,7, 4'-tetrahydroxyflavone) were identified. Their contents, and relative amounts, in the analyzed honey samples
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 10357 | 04061835551026 |
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I want to isolate the flavonoids from the plant extract. I need 100g of Amberlite XAD 2. Can you provide it? can i use this isolation of flavonoids
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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Does Amberlite XAD-2 work as a sorbent for VOCs with further thermodesorption to GC-FID?
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The Amberlite™ XAD®-2 sorbent has been used for the adsorption of volatile organic compounds from both aqueous and organic solvents, as well as from gas-phase samples. Thermal desorption is possible for GC-FID analysis; however, temperature limitations should be noted. Volatile residues may be emitted from the resin at temperatures above 150 °C, and the resin will begin to pyrolyze at 250 °C. For applications requiring higher temperatures, a graphitized carbon sorbent is recommended.
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What is the most approriate protocol to wash Amberlite before use? Thanks in advance!
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Amberlite XAD-2 is a crude preparation and must be thoroughly cleaned prior to use.
1. Rinse with hot deionized water (may hold in nylon filter cloth to retain particles) - use at least 3 bed volumes per hour at 110 deg C
2. Soak overnight in 2 bed volumes (BV) of ethanol.
3. Load resin into a glass column and rinsed with a further 2 BV of ethanol.
4. Rinse with 2 BV of deionized water to remove the ethanol, 1 BV of 4% (w/v) sodium hydroxide, 2 BV of deionized water, 1 BV 4% (v/v) hydrochloric acid, and finally rinse with deionized water until the pH of the eluent is neutral.For further use it is advantageous to do a wash in the same solvent use in the application.
Store in sealed container; may be refrigerated but NOT frozen. As a bacteriostat, one may rinse with 20% ethanol (deionized water) prior to storage.Helpful?
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Do you offer a precleaned Amberlite XAD-2?
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Yes. Supelco cleans Amberlite XAD2 for a variety of environmental applications.
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May I purchase Amberlite XAD-2 in large quantities?
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The largest package size of Amberlite XAD2 is 25 kg (Product No. 3025-U).
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Is Amberlite XAD-2 ready-to-use?
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No. Amberlite XAD2 is shipped imbibed with 5% NaCl & 1% NaCO3; it has not been specially processed nor cleaned. We suggest that it be extensively treated to a suitable preliminary elution and wash.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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