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U0631

Urea

BioXtra, pH 7.5-9.5 (20 °C, 5 M in H2O)

Synonym(s):

Carbamide, Carbonyldiamide

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About This Item

Linear Formula:
NH2CONH2
CAS Number:
Molecular Weight:
60.06
UNSPSC Code:
12352111
NACRES:
NA.31
PubChem Substance ID:
EC Number:
200-315-5
Beilstein/REAXYS Number:
635724
MDL number:
Assay:
98.5-101.5%
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product line

BioXtra

Quality Segment

assay

98.5-101.5%

form

powder, crystals or pellets

impurities

≤0.005% Phosphorus (P), ≤0.1% Insoluble matter

ign. residue

≤0.1%

pH

7.5-9.5 (20 °C, 5 M in H2O)

mp

132-135 °C (lit.)

solubility

H2O: 5 M at 20 °C, clear, colorless

density

1.335 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤0.05%, sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.0005%, Ca: ≤0.001%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Mg: ≤0.0005%, NH4+: ≤0.05%, Na: ≤0.005%, Pb: ≤0.001%, Zn: ≤0.0005%

absorption

≤0.06 at 260 nm at 5 M, ≤0.06 at 280 nm at 5 M

application(s)

diagnostic assay manufacturing

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Gene Information

human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

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This Item
U53781.370301.04166
Urea crystalline, EMPROVE® EXPERT, Ph. Eur., BP, ChP, JP, USP, ACS

SAFC

1.37030

Urea

Urea granulated, EMPROVE® EXPERT, Ph. Eur., BP, ChP, JP, USP, ACS

SAFC

1.04166

Urea

assay

98.5-101.5%

assay

≥98%

assay

98.0-102.0% (HPLC)

assay

-

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

liquid formulation
parenterals
pharma/biopharma processes
pharmaceutical

application(s)

liquid formulation
parenterals
pharma/biopharma processes
pharmaceutical

Quality Level

200

Quality Level

200

Quality Level

500

Quality Level

500

solubility

H2O: 5 M at 20 °C, clear, colorless

solubility

H2O: soluble 480 mg/mL, clear, colorless (8M)

solubility

-

solubility

-

form

powder, crystals or pellets

form

powder

form

crystalline, solid

form

granular

pH

7.5-9.5 (20 °C, 5 M in H2O)

pH

-

pH

9 (20 °C, 100 g/L in H2O)

pH

9


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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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