T8453

Anti-β-Tubulin II antibody, Mouse monoclonal

clone 7B9, purified from hybridoma cell culture

• Synonym(s): Anti-TUBB2, Anti-Tubulin, β2
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00145892
• Conjugate: unconjugated
• Clone: 7B9, monoclonal
• Application: ICC, IP, WB
• Citations: 12

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 7B9, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~50 kDa
• species reactivity: human, mouse, rat
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): suitable, western blot: 1-2 μg/mL using Neuro-2a cell extract, immunocytochemistry: suitable
• isotype: IgG1
• UniProt accession no.: Q9BVA1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TUBB2B(347733) ; mouse ... Tubb2b(73710) ; rat ... Tubb2b(291081)

Description

General description

Monoclonal Anti- β-Tubulin II (mouse IgG1 isotype) is derived from the hybridoma 7B9 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment at the C-terminus of human β?tubulin II. Tubulin is the major building block of microtubules. This intracellular, cylindrical, filamentous structure is present in almost all eukaryotic cells. Tubulin is a heterodimer that consists of α-tubulin and β ?tubulin. Both subunits have a molecular mass of ~50 kDa and share considerable homology.

Application

Anti-β-Tubulin II antibody has been used in

• immunoblotting immunoprecipitation
• immunocytochemistry
• western blotting
• immunolabelling

Biochem/physiol Actions

The αβ tubulins are involved in the formation of microtubules during cell division by the formation of protofilaments.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)