T7039

Transforming Growth Factor-β1 human

TGF-β1, recombinant, expressed in CHO cells, powder, suitable for cell culture

• Synonym(s): hTGF-β1, TGF-β1
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00166089
• Biological source: human
• Recombinant: expressed in CHO cells
• Assay: ≥95% (SDS-PAGE)
• Form: powder
• Mol wt: dimer (12.8/25.6 kDa (112/224 aa))
• Impurities: ≤0.1 EU/μg (LAL test)

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in CHO cells
• assay: ≥95% (SDS-PAGE)
• form: powder
• potency: ≤0.5 ng/mL ED₅₀
• mol wt: dimer (12.8/25.6 kDa (112/224 aa))
• packaging: pkg of 2 μg, pkg of 50 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤0.1 EU/μg (LAL test)
• UniProt accession no.: P01137
• storage temp.: −20°C

Description

General description

Tgfb1 (transforming growth factor-β1 human) is a 25kDa peptide encoded by the gene mapped to human chromosome 19q13.2. TGF-β1 polypeptide is produced as a precursor with hydrophobic signal sequence, pro-region and mature peptide. Cleavage of the precursor at tetrabasic cleavage site results in monomers of carboxy-terminal 112 amino acids. The biologically active form of Tgfb1 exists as a disulphide-linked homodimer. In mammals, three isoforms of TGF-β has been identified : TGF-β1, TGF-β2 and TGF-β3.

Application

Transforming Growth Factor-β1 human has been used in

• reporter gene assay.[1]
• in vitro transfection.
• to study the effect of transforming growth factor-β1 (TGF-β1) on gene array analysis of renal cells.
• to study the association of TGF-β1 with shear stress-dependent upregulation of tissue inhibitor of metalloproteinase-1 in microvascular endothelial cells.
• to study the interaction between TGF-β1 activation and myofibroblast formation.

Biochem/physiol Actions

Transforming growth factor-β1 (TGF-β1) is produced by many cell types, but is reported to be most concentrated in mammalian platelets, where it is present at approximately four times the level of TGF-β2.

TGF-β1 is a multifunctional peptide capable of influencing cell proliferation, tissue growth, differentiation, and other functions in a wide range of cell types. TGF-β1 plays a fundamental role in differentiation by involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function. All cells possess a specific TGF-β1 receptor. Both transformed and non-neoplastic tissues release transforming growth factors.The multi-modal nature of TGF-β1 is seen in its ability to stimulate or inhibit cellular proliferation. TGF-β1 is associated with the developmental and metastasis of colorectal cancer.

Physical form

Lyophilized from a 0.2 µm filtered solution containing 0.1 % trifluoroacetic acid (TFA) and Trehalose in a 20:1 Trehalose to protein ratio

Preparation Note

Reconstitution: Sterile 10 mM HCl at 0.1 mg/mL

Analysis Note

The biological activity of TGF-β1 is measured in culture by inhibition of mouse IL-4-dependent proliferation of mouse HT-2 cells.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)