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T7024

N,N,N′,N′-Tetramethylethylenediamine

BioReagent, Molecular Biology, ≥99% (GC)

Synonym(s):

1,2-Bis(dimethylamino)ethane, TEMED, TMEDA

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About This Item

Linear Formula:
(CH3)2NCH2CH2N(CH3)2
CAS Number:
Molecular Weight:
116.20
UNSPSC Code:
41105319
NACRES:
NA.31
PubChem Substance ID:
EC Number:
203-744-6
Beilstein/REAXYS Number:
1732991
MDL number:
Assay:
≥99% (GC)
Grade:
Molecular Biology
Technique(s):
electrophoresis: suitable
Bp:
120-122 °C (lit.)
Vapor pressure:
ca.19.19 hPa
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grade

Molecular Biology

vapor pressure

ca.19.19 hPa

product line

BioReagent

assay

≥99% (GC)

expl. lim.

9.08 %

technique(s)

electrophoresis: suitable

refractive index

n20/D 1.4179 (lit.)

bp

120-122 °C (lit.)

mp

−55 °C (lit.)

density

0.775 g/mL at 20 °C (lit.)

foreign activity

DNase, RNase, protease, none detected

SMILES string

CN(C)CCN(C)C

InChI

1S/C6H16N2/c1-7(2)5-6-8(3)4/h5-6H2,1-4H3

InChI key

KWYHDKDOAIKMQN-UHFFFAOYSA-N

Application

This product is designated as molecular biology grade and is suitable for polyacrylamide gel electrophoresis (PAGE). It has been analyzed for the absence of nucleases and proteases.
TEMED is a tertiary amine that is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. TEMED-generated free radicals cause the compounds acrylamide and bis-acrylamide to polymerize and form the gel matrix polyacrylamide. Because TEMED is functional only in its free base form, low pH conditions will inhibit polymerization.[1],,
The potential contribution of TEMED to elevated background in silver stained SDS PAGE has been studied.[2] TEMED has been used in the coating of soft polymers, such as polyacrylamide, in capillaries for investigation by atomic force microscopy.[3] Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED.[4]
The capillary isoelectric focusing of proteins with an isoelectric point (pI) > 12 has been investigated through the use of TEMED.[5] TEMED has been used in the HPLC of small organic molecules.[6]

Preparation Note

Suggested use is 35 μl for 50 ml acrylamide gel solution.

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This Item
T9281T225001.10732
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

assay

≥99% (GC)

assay

≥99.0%

assay

99%

assay

≥99% (GC)

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

-

grade

Molecular Biology

grade

-

grade

-

grade

-

mp

−55 °C (lit.)

mp

−55 °C (lit.)

mp

−55 °C (lit.)

mp

-55 °C

refractive index

n20/D 1.4179 (lit.)

refractive index

n20/D 1.4179 (lit.)

refractive index

n20/D 1.4179 (lit.)

refractive index

-


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signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B

Storage Class

3 - Flammable liquids

wgk

WGK 1

flash_point_f

61.7 °F - closed cup

flash_point_c

16.5 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter



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Andrews, A. T.
Electrophoresis: Theory, Techniques, and Biochemical and Clinical Applications, 21-24 (1986)
A V Vasiliskov et al.
BioTechniques, 27(3), 592-594 (1999-09-18)
The manufacturing of microchips containing oligonucleotides and proteins immobilized within gel pads, ranging in size from 10 x 10 to 100 x 100 microns, is described. The microchips are produced by photo- or persulfate-induced copolymerization of unsaturated derivatives of biomolecules
Sambrook, J. and Russell, D. W.
Molecular Cloning: A Laboratory Manual, 5-5 (2001)



Global Trade Item Number

SKUGTIN
T7024-50ML04061838260321
T7024-25ML04061838260314
T7024-100ML04061838260307

Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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