T6793

Monoclonal Anti-Tubulin, Acetylated antibody produced in mouse

clone 6-11B-1, ascites fluid

• Synonym(s): Anti-ALS22, Anti-H2-ALPHA, Anti-TUBA1
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00164512
• Conjugate: unconjugated
• Clone: 6-11B-1, monoclonal
• Application: DB, ELISA (i), EM, ICC, RIA, WB
• Citations: 1333

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 6-11B-1, monoclonal
• contains: 15 mM sodium azide
• species reactivity: mouse, protista, invertebrates, hamster, plant, frog, rat, monkey, pig, bovine, chicken, human
• technique(s): dot blot: suitable, electron microscopy: suitable, immunocytochemistry: suitable, indirect ELISA: suitable, radioimmunoassay: suitable, western blot: 1:2,000 using a bovine or rat brain preparation
• isotype: IgG2b
• UniProt accession no.: P68366
• application(s): research pathology
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TUBA4A(7277) ; mouse ... Tuba1a(22142) ; rat ... Tuba1a(64158)

Description

General description

α-tubulin, also called as tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α-tubulin family containing 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000.

Immunogen

acetylated α-tubulin from the outer arm of sea urchin sperm axonemes.

Application

Monoclonal Anti-Tubulin, Acetylated antibody produced in mouse has been used in following studies:

• immunofluorescence.
• western blotting.[1]
• immunocytochemistry.
• immunohistochemistry.[2]

Biochem/physiol Actions

Acetylated tubulin is associated with axoneme assembly in a variety of organisms. Acetylated tubulin might play a crucial role in the differentiation of microtubule structure and function. Post translational modification of α-tubulin i.e. the reversible addition of an acetyl group to Lys40, by specific acetylase, results in acetylated tubulin.

The antibody recognizes an epitope located on the α3 isoform of Chlamydomonas axonemal α-tubulin, within four residues of Lys-40 when this amino acid is acetylated. A sequence very similar to the one detected by the antibody in Chlamydomonas is found in the majority of α-tubulins, but the corresponding region is markedly divergent in some α-tubulin isoforms from chicken, Drosophila and yeast. The antibody has been used to detect acetylated α-tubulins in several organisms. Occasionally, the epitope recognized by the antibody may be absent or masked as it is in the kangaroo rat epithelial-like cell line PtK₂.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable