T3449

TrypZean^® Solution, 1×

recombinant, expressed in corn, sterile-filtered

• Synonym(s): Trypsin
• NACRES: NA.75
• UNSPSC Code: 12352204
• Recombinant: expressed in corn
• Concentration: 1 ×

Properties

• recombinant: expressed in corn
• Quality Segment: 300
• sterility: sterile-filtered
• form: aqueous solution
• concentration: 1 ×
• technique(s): cell culture | mammalian: suitable
• pH: 7.70-8.30
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

TrypZean^® solution, 1× is formulated with TrypZean, recombinant bovine trypsin expressed in corn and manufactured utilizing ProdiGene′s proprietary transgenic plant protein expression system. This product is optimized for cell dissociation in both serum-free and serum-supplemented adherent cell cultures.

Application

TrypZean^® Solution, 1× has been used:

• for the suspension of pellet during DNA extraction[1]
• digestion of cells to single-cell suspension for pharmacotyping of 2D cells[2]
• as recombinant trypsin to induce cell division of Vero cells sub cultivated in IPT-AF medium[3]

Features and Benefits

TrypZean^® Solution provides the following features and benefits:

• Animal component-free – eliminates the risk of viruses, BSE, or other potential adventitious agents
• TrypZean is recombinant trypsin – using the same enzyme (with the same kinetics) for cell detachment means minimal protocol changes
• Enzyme inhibition – soybean trypsin inhibitor and other inhibitors work the same with TrypZean as they do with native trypsins (weight-to-weight basis)
• High purity – TrypZean provides increased specificity and eliminates contaminating activities found in lower purity enzymes
• Convenience – TrypZean solution is a sterile-filtered solution formulated at the optimal concentration to dissociate adherent cell lines

Legal Information

Trypzean is a registered trademark of ProdiGene, Inc.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable