The product with code SAE0097 contains a His tag.
SAE0097
D-2-Hydroxyglutarate Dehydrogenase (D2HGDH) from Acidaminococcus fermentans
recombinant, expressed in E. coli, aqueous solution
Synonym(s):
D2HGDH, HGDH, L-2-hydroxyglutarate dehydrogenase
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About This Item
EC Number:
1.1. 99.2
NACRES:
NA.54
UNSPSC Code:
12352202
Specific activity:
≥1000 units/mg protein
Assay:
≥95% (SDS-PAGE)
Recombinant:
expressed in E. coli
Pricing and availability is not currently available.
General description
D-2-Hydroxyglutarate Dehydrogenase (D2HGDH) is a member of the D-2-hydroxyacid NAD+ dependent dehydrogenase family of proteins. D2HGDH catalyzes the conversion of α-ketoglutarate (α--KG) to D-2-hydroxyglutarate (D2HG), coupled to the oxidation of NADH to NAD+ .
The crystal structure of D2HGDH from Acidaminococcus fermentans has been reported. D2HGDH from Acidaminococcus fermentans has been used in several enzymatic assays, such as:
The crystal structure of D2HGDH from Acidaminococcus fermentans has been reported. D2HGDH from Acidaminococcus fermentans has been used in several enzymatic assays, such as:
- A continuous spectrophotometric assay to measure the activity of aminotransferases, based on the transamination of a keto compound and L-glutamate, which yields a corresponding amino compound and 2-oxoglutarate.
- Determination of D2HG levels in biological fluids such as serum, urine, cell culture supernatants, and cell or tissue lysates.
- A coupled assay system to measure branched-chain amino acid aminotransferase activity.
Preparation Note
This recombinant D2HGDH product is supplied as an aqueous solution in 20 mM Trizma® buffer, pH 7.5, with 150 mM NaCl, and 10% glycerol.
Other Notes
One unit of enzyme oxidizes 1 μmole of NADH to NAD+ coupled to the reduction of α-ketoglutarate to (D)-2-hydroxyglutarate per minute at 37°C at pH 8.0.
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| assay ≥95% (SDS-PAGE) | assay - | assay - | assay - |
| specific activity ≥1000 units/mg protein | specific activity 100-300 units/mg protein | specific activity ≥250 units/mg protein (biuret) | specific activity 0.1-1.0 units/mg protein (Lowry) |
| form aqueous solution | form ammonium sulfate suspension | form ammonium sulfate suspension | form buffered aqueous glycerol solution |
| recombinant expressed in E. coli | recombinant - | recombinant - | recombinant - |
| UniProt accession no. | UniProt accession no. - | UniProt accession no. - | UniProt accession no. - |
| shipped in wet ice | shipped in - | shipped in - | shipped in dry ice |
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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