Unfortunately, the formulation of Product S5442 is considered proprietary.
S5442
SyntheChol® NS0 Supplement
500 ×, synthetic cholesterol, animal component-free, sterile-filtered, aqueous solution, suitable for cell culture
SyntheChol® NS0 Supplement
Synonym(s):
Cholesterol solution
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About This Item
Empirical Formula (Hill Notation):
C27H46O
CAS Number:
Molecular Weight:
386.65
UNSPSC Code:
12352211
eCl@ss:
39023139
PubChem Substance ID:
NACRES:
NA.75
MDL number:
Pricing and availability is not currently available.
biological source
non-animal source
Quality Level
sterility
sterile-filtered
product line
BioXtra
form
aqueous solution
concentration
500 ×
technique(s)
cell culture | mammalian: suitable
storage temp.
2-8°C
SMILES string
[H][C@@]12CC=C3C[C@@H](O)CC[C@]3(C)[C@@]1([H])CC[C@]4(C)[C@H](CC[C@@]24[H])[C@H](C)CCCC(C)C
InChI
1S/C27H46O/c1-18(2)7-6-8-19(3)23-11-12-24-22-10-9-20-17-21(28)13-15-26(20,4)25(22)14-16-27(23,24)5/h9,18-19,21-25,28H,6-8,10-17H2,1-5H3/t19-,21+,22+,23-,24+,25+,26+,27-/m1/s1
InChI key
HVYWMOMLDIMFJA-DPAQBDIFSA-N
Gene Information
human ... POLA1(5422), TOP2A(7153)
rat ... Ar(24208), Polb(29240)
Application
Features and Benefits
Synthetic, non-animal cholesterol solution that is designed as a supplement to our hybridoma media formulations to promote excellent cell growth and protein productivity for NS0 mouse myeloma and other cholesterol-dependent cell lines.
Legal Information
SyntheChol is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - |
| biological source non-animal source | biological source ovine wool | biological source sheep wool | biological source synthetic |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. room temp | storage temp. 15-25°C |
| form aqueous solution | form powder | form powder | form - |
| concentration 500 × | concentration - | concentration - | concentration - |
| Quality Level 500 | Quality Level 200 | Quality Level 200 | Quality Level 500 |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| S5442-2ML | 04061836954444 |
| S5442-10ML | 04061836954222 |
| S5442-100ML | 04061838446916 |
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What is the concentration of product S5442?
1 answer-
Helpful?
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Is this formulation stable at room temperature? If so, how long is it stable?
1 answer-
This product ships at ambient temperature and is stable for relatively short periods (up to two weeks for transit time) at room temperature. However, refrigerated temperatures are recommended for long-term storage.
Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/397/094/s5442dat.pdfHelpful?
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Could you please clarify the concentration of cholesterol in this product both at the original 500X solution and at the final 1X?
1 answer-
The concentration of synthetic cholesterol in this product is 0.25% or 2.5 mg/mL as supplied. The product is considered a 500X preparation meaning that 2 mL of this solution will supplement 1 L of culture medium. The 1X concentration, when diluted in 1L of medium, would be 5 ug/mL. Some cells may require a higher concentrations of cholesterol. The suggested usage range for this product is 2 - 6 mL per liter of medium. See the link below to review the product datasheet.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/397/094/s5442dat.pdfHelpful?
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Hi, What is the concentration of Synthechol in this product in mg/ml or mol/l Thank you
1 answer-
Unfortunately, the formulation of item S5442 is proprietary. The concentration of the synthetic cholesterol is 0.25% (w/v) as supplied.
Helpful?
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