S2628

N-Succinyl-L-phenylalanine-p-nitroanilide

protease substrate, ≥98% (TLC), powder

• Synonym(s): Suc-Phe-pNA
• Empirical Formula (Hill Notation): C₁₉H₁₉N₃O₆
• CAS Number: 2440-62-2
• Molecular Weight: 385.37
• NACRES: NA.32
• PubChem Substance ID: 24899530
• UNSPSC Code: 12352204
• EC Number: 219-472-6
• MDL number: MFCD00047721

Properties

• Product Name: N-Succinyl-L-phenylalanine-p-nitroanilide, protease substrate
• Quality Segment: 200
• assay: ≥98% (TLC)
• form: powder
• solubility: dioxane: 25 mg/mL, clear, colorless to faintly yellow
• storage temp.: −20°C
• SMILES string: OC(=O)CCC(=O)N[C@@H](Cc1ccccc1)C(=O)Nc2ccc(cc2)N(=O)=O
• InChI: 1S/C19H19N3O6/c23-17(10-11-18(24)25)21-16(12-13-4-2-1-3-5-13)19(26)20-14-6-8-15(9-7-14)22(27)28/h1-9,16H,10-12H2,(H,20,26)(H,21,23)(H,24,25)/t16-/m0/s1
• InChI key: KNBLWBFJHZHYFG-INIZCTEOSA-N

Description

General description

Reported to be a substrate for chymotrypsin.

Application

N-Succinyl-L-phenylalanine-p-nitroanilide has been used as a substrate for the hydrolytic activity of chymotrypsin and molar absorptive experiments. It has also been used for monitoring in vitro α-chymotrypsin inhibition assay.

Biochem/physiol Actions

N-Succinyl-L-phenylalanine-p-nitroanilide, a substrate for the hydrolytic activity of chymotrypsin, forms a yellow chromophore, p-nitroaniline, which can be measured spectrophotometrically at 410 nm.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)