RDD008

Trizma^® base

≥99.9%, powder, anhydrous, Redi-Dri^™

• Synonym(s): 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
• Linear Formula: NH₂C(CH₂OH)₃
• CAS Number: 77-86-1
• Molecular Weight: 121.14
• NACRES: NA.25
• PubChem Substance ID: 329824219
• UNSPSC Code: 12161700
• EC Number: 201-064-4
• MDL number: MFCD00004679
• Beilstein/REAXYS Number: 741883

Properties

• Product Name: Trizma^® base, anhydrous, free-flowing, Redi-Dri^™, ≥99.9%
• grade: anhydrous
• Quality Level: 100
• description: aminopeptidase substrate
• product line: Redi-Dri^™
• assay: ≥99.9%
• form: powder
• quality: free-flowing
• does not contain: Carbon Fiber
• impurities: ≤0.2% Water (Karl Fischer)
• color: white
• pH: 10.5-12, 7-9
• useful pH range: 7-9
• pKa (25 °C): 8.1
• bp: 219-220 °C/10 mmHg (lit.)
• mp: 167-172 °C (lit.)
• solubility: water: soluble (Water < 0.2%)
• absorption: ≤0.05 at 290 nm at 40%
• λ: (A290 UV absorbance < 0.05), in 5 mM Tris; DNA (40% (w/w))
• SMILES string: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• InChI key: LENZDBCJOHFCAS-UHFFFAOYSA-N

Description

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Tris base has been used:

• To prepare Tris-HCl, which is used as a component of lysis buffer for protein characterization
• In the preparation of Tris-buffered saline Tween-20 (TBST) buffer for Western blotting
• In the preparation of TBS (Tris-buffered saline) that is used for dialysis to neutralize a protein
• As a component of nuclei extraction buffer

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable