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Protease from Bacillus licheniformis

Name: Protease from <I>Bacillus licheniformis</I>
Brand: SIGMA

glycerol solution (50%)

Synonym(s):

Proteinase from Bacillus licheniformis, Subtilisin A

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About This Item

CAS Number:

9014-01-1

UNSPSC Code:

12352204

eCl@ss:

32160410

NACRES:

NA.54

EC Number:

3.4.21.62 (BRENDA, IUBMB)

MDL number:

MFCD00132092

Specific activity:

≥7 units/mg protein (biuret)

Biological source:

Bacillus licheniformis

Concentration:

≥50 mg/mL

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Properties

biological source

Bacillus licheniformis

Quality Segment

200

form

glycerol solution (50%)

specific activity

≥7 units/mg protein (biuret)

concentration

≥50 mg/mL

solubility

0.85% sodium chloride: soluble, clear (Diluted), H₂O: miscible

storage temp.

2-8°C

Description

General description

The enzyme hydrolyzes gelatin optimally at 50 °C over the range of pH 8-10. It is stable between pH 6-10 at 50 °C. Commercially, it is used in laundry detergent formulations and also for the recovery of silver from photographic emulsions.

Application

Protease from Bacillus licheniformis has been used to digest the neurolemma in the antennal lobe of the moth; Heliothis virescens.[1]

Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations. Protease from Bacillus licheniformis, product P5459, has been used to hydrolyze muscle during the isolation of mitochondria from skeletal muscle.

Biochem/physiol Actions

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate.

Packaging

Package size based on protein content.

Other Notes

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

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This Item	P5380	P4860	126741
Sigma-Aldrich P5459 Protease from Bacillus licheniformis	Sigma-Aldrich P5380 Protease from Bacillus licheniformis	Sigma-Aldrich P4860 Protease from Bacillus licheniformis	Sigma-Aldrich 126741 Alcalase^® Enzyme, Bacillus licheniformis
biological source Bacillus licheniformis	biological source Bacillus licheniformis	biological source -	biological source Bacillus licheniformis
specific activity ≥7 units/mg protein (biuret)	specific activity 7-15 units/mg solid	specific activity ≥2.4 U/g	specific activity ≥0.75 Anson units/mL
form glycerol solution (50%)	form lyophilized powder	form aqueous solution	form liquid
concentration ≥50 mg/mL	concentration -	concentration -	concentration -
storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C
solubility 0.85% sodium chloride: soluble, clear (Diluted), H₂O: miscible	solubility 10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble, clear, H₂O: soluble, colorless	solubility -	solubility -