N5661

Nuclease S₁ from Aspergillus oryzae

for single-strand DNA/RNA digestion

• Synonym(s): Endonuclease S₁
• CAS Number: 37288-25-8
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 3.1.30.1 (BRENDA, IUBMB)
• MDL number: MFCD00131010
• Biological source: Aspergillus sp. (A. oryzae)
• Concentration: ≥100000 units/mL

Properties

• biological source: Aspergillus sp. (A. oryzae)
• Quality Level: 200
• form: solution
• concentration: ≥100000 units/mL
• technique(s): DNA purification: suitable
• suitability: suitable for nucleic acid purification
• application(s): cell analysis
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

The Nuclease S1 enzyme from Aspergillus oryzae has the ability to degrade single-stranded oligonucleotides composed of either deoxynucleotides or ribonucleotides. [1]

Application

Nuclease S1 from Aspergillus oryzae has been used in a study to assess a biochemical method for mapping mutational alterations in DNA. [2] It has also been used in a study to investigate the DNA damage and repair in a γ-irradiated rat brain tumor. [3]

Biochem/physiol Actions

Nuclease S1 isolated from Aspergillus oryzae exhibits endo- and exolytic hydrolytic activity for the phosphodiester bonds of single-stranded DNA and RNA yielding 5′-phosphomononucleotide and 5′-phosphooligonucleotide end-products. It is used to digest non-annealed polynucleotide tails and hairpin loops in RNA and DNA duplexes and can be used to convert superhelical DNA to the linear form.

SI nuclease from Aspergillus oryzae can generate double-stranded DNA breaks in response to DNA nicks or abasic sites. [4]

Physical form

Solution containing 30 mM sodium acetate, 50 mM NaCl, 1 mM ZnCl₂, 50% glycerol, 2 mg/ml protein

Other Notes

One unit will cause 1.0 microgram of single-stranded nucleic acid to become perchloric acid soluble per minute at pH 4.6 at 37°C.

Kit Components Only

• 30mM Sodium acetate .25-.25 %
• 50mM Sodium chloride .29 %
• 1mM Zinc chloride .01 %
• Glycerol 50 %
• 2mg/mL Protein .2 %

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable