This kit was originally developed for testing mammalian serum, plasma or cultured cell lines. The assay buffer that comes with the kit is at pH 10.5. The pH optimum for bacterial alkaline phosphatase (BAP) from E. coli has been reported to be 8.5, but it would still have activity at that higher pH. So while the kit has not been optimized for BAP, it would be able to detect activity from E. coli cell lysates.
MAK447
Alkaline Phosphatase Assay Kit
Sufficient for 250 colorimetric tests
Synonym(s):
ALP Activity Assay Kit, Alkaline Phosphatase Test Kit
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
Pricing and availability is not currently available.
Quality Segment
application(s)
life science and biopharma
detection method
colorimetric
relevant disease(s)
osteoporosis; gastrointestinal diseases; orthopedic diseases; cancer
storage temp.
−20°C
General description
Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis.
Application
The Alkalnine Phosphatase Assay Kit may be used for:
- Cancer Research
- Gastroenterology Disease Research
- Osteoporosis Research
The kit is suitable for the quantitative determination of alkaline phosphatase (ALP) activity in serum, plasma etc.
Biochem/physiol Actions
The Alkaline Phosphatase Assay Kit is designed to measure ALP activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl phosphate that is hydrolyzed by ALP into a yellow colored product (maximal absorbance at A405 nm). The rate of the reaction is directly proportional to the enzyme activity.
Features and Benefits
Enhanced Detection Range: Detect ALP levels accurately across a wide range, from 2 U/L to 800 U/L, using only 5 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.
Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a short 5 minute assay reaction, reducing complexity and saving valuable time and effort.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a short 5 minute assay reaction, reducing complexity and saving valuable time and effort.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
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This Item | |||
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| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| detection method colorimetric | detection method - | detection method - | detection method colorimetric |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| application(s) life science and biopharma | application(s) - | application(s) - | application(s) - |
| relevant disease(s) osteoporosis; gastrointestinal diseases; orthopedic diseases; cancer | relevant disease(s) - | relevant disease(s) - | relevant disease(s) cancer, prostate tumor; gastrointestinal diseases |
signalword
Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
target_organs
Nervous system,Kidney,Liver,Skin
Storage Class
8A - Combustible corrosive hazardous materials
wgk
WGK 3
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Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAK447-1KT | 04065267335636 |
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Can the Alkaline Phosphatase Assay Kit (MAK447) be used on E. coli cell lysates?
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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