The extraction solvent included with this kit contains 0.15 mg of internal standard for each 3 mL aliquot added to a sample. The Product Information Sheet included with this kit provides instructions on the usage of this internal standard in the determination of sample lipid concentration from GC-FID results.
MAK174
Fatty Acid Extraction Kit, Low Standard
sufficient for 40 extractions
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About This Item
NACRES:
NA.25
UNSPSC Code:
12161503
Pricing and availability is not currently available.
usage
sufficient for 40 extractions
greener alternative product characteristics
Waste Prevention
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
application(s)
cosmetics
food and beverages
sample preservation
greener alternative category
, Aligned
relevant disease(s)
cancer
storage temp.
room temp
General description
Lipids are structural components of cell membranes that play a critical role in gene transcription, signaling, and metabolism. Several lipid species exist in biological systems, including phospholipids, triglycerides, and fatty acids. When extracting fatty acids at low or very low levels, such as in cellular matrices, use the MAK174 fatty acid extraction kit. When extracting fatty acids at normal or high levels, such as in food matrices, use the MAK338 fatty acid extraction kit.
The Folch method has been conventionally used to extract lipids containing fatty acids from biological samples, using chloroform, methanol, and water to separate lipids from aqueous-soluble compounds. In this procedure, lipids are retained in the lower chloroform layer; whereas, aqueous-soluble compounds are retained in the upper methanol-water layer. The sample is then centrifuged to achieve uniform separation and the bottom chloroform layer is transferred with a pipette to a new test tube.
The Folch method has been conventionally used to extract lipids containing fatty acids from biological samples, using chloroform, methanol, and water to separate lipids from aqueous-soluble compounds. In this procedure, lipids are retained in the lower chloroform layer; whereas, aqueous-soluble compounds are retained in the upper methanol-water layer. The sample is then centrifuged to achieve uniform separation and the bottom chloroform layer is transferred with a pipette to a new test tube.
Application
Fatty Acid Extraction Kit has been used for fatty acid extraction.[1]
This kit is suitable for the extraction of lipids from a variety of samples such as whole blood, serum, biological tissue, food samples, and oil.
Biochem/physiol Actions
The Fatty Acid Extraction Kit shortens this extraction process by eliminating the need to prepare solvents and standards, centrifugation, and pipetting. Once the sample is homogenized and dissolved in the Extraction Solvent supplied in our kit, it is inverted twice and poured into the syringe containing a filter, which preferentially elutes the chloroform layer containing total lipids. A portion of the total lipid extract containing fatty acids can then be transesterified for GC-FID analysis.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product reduces solvent waste over traditional methods. Find details here.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product reduces solvent waste over traditional methods. Find details here.
Analysis Note
Case study can be found here: Experts have tested the Fatty Acid Extraction Kit: Time savings and greener alternative
Other Notes
Experts have tested the Fatty Acid Extraction Kit. Watch the Video to learn more and to see how the technology works.
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This Item | |||
|---|---|---|---|
| usage sufficient for 40 extractions | usage sufficient for 40 extractions | usage sufficient for 40 extractions | usage sufficient for 100 fluorometric tests |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. −20°C |
| relevant disease(s) cancer | relevant disease(s) cancer | relevant disease(s) - | relevant disease(s) endocrinological disorders, diabetes; gastrointestinal diseases; cancer |
| application(s) cosmetics | application(s) cosmetics | application(s) cosmetics | application(s) - |
| greener alternative product characteristics Waste Prevention | greener alternative product characteristics Waste Prevention | greener alternative product characteristics Waste Prevention | greener alternative product characteristics - |
| greener alternative category , Aligned | greener alternative category | greener alternative category | greener alternative category - |
signalword
Danger
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Flam. Liq. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 1 - STOT SE 3
target_organs
Central nervous system, Eyes, Liver,Kidney
Storage Class
3 - Flammable liquids
flash_point_f
49.5 °F - closed cup
flash_point_c
9.7 °C - closed cup
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Related Content
Zhichao Zhang et al.
Journal of food science, 82(10), 2291-2297 (2017-08-24)
Fatty acid extraction methods are time-consuming and expensive because they involve multiple steps and copious amounts of extraction solvents. In an effort to streamline the fatty acid extraction process, this study compared the standard Folch lipid extraction method to a
Structure of the Plasma Membrane
Cooper G M.
The Cell: A Molecular Approach (2000)
Liquid Extraction: Folch
Markus R W
Encyclopedia of Lipidomics (2016)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAK174-1KT | 04061834065654 |
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Does the MAK174 contain internal standard and how much is it?
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