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Malic Dehydrogenase from porcine heart

Name: Malic Dehydrogenase from porcine heart
Brand: SIGMA

≥600 units/mg protein (biuret), ammonium sulfate suspension

Synonym(s):

L-Malate: NAD⁺ oxidoreductase, MDH, Malate Dehydrogenase

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-622-5

MDL number:

MFCD00131567

EC Number:

1.1.1.37 (BRENDA, IUBMB)

Specific activity:

≥600 units/mg protein (biuret)

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Properties

form

ammonium sulfate suspension

Quality Segment

200

specific activity

≥600 units/mg protein (biuret)

foreign activity

Glutamic-Oxalacetic Transaminase ≤0.01%, Glutamic-Pyruvic Transaminase ≤0.01%

storage temp.

2-8°C

Description

General description

Malic dehydrogenase exists as a dimer with each subunit containing an NAD-binding domain and a substrate-binding carboxy-terminal domain required for activity. [1]

Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.

Malic Dehydrogenase is a ubiquitous enzyme, which exists in two isoforms in eukaryotic cells.

Application

Malic dehydrogenase has been used in a study to assess the effect of an immunomodulator S2 complex on the enzymes of the parasites. [2] It has also been used in a study to investigate the heterogeneity of lactic and malic dehydrogenase in cerebrospinal fluid.

Malic Dehydrogenase from porcine heart has been used:

in qualitative protein binding measurements
to test internally calibrated electrochemical continuous enzyme assay (ICECEA) with model enzyme pair
to investigate the effect of chaperone on the refolding of heat-denatured malate dehydrogenase

Biochem/physiol Actions

Malic Dehydrogenase (MDH) plays an important role in the citric acid cycle in mitochondria. It catalyzes the interconversion of substrates malate and oxaloacetate with the simultaneous oxidation/reduction of NAD/NADH⁺. MDH present in the cytosol is involved in the shuttling of malate/aspartate.

Physical form

Suspension in 2.8 M (NH₄)₂SO₄ solution, pH 6.0

Other Notes

One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25°C.

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This Item	M7383	M2634	M9004
Sigma-Aldrich M1567 Malic Dehydrogenase from porcine heart	Sigma-Aldrich M7383 Malic Dehydrogenase from porcine heart	Sigma-Aldrich M2634 Malic Dehydrogenase from porcine heart	Sigma-Aldrich M9004 Malic Dehydrogenase from bovine heart
specific activity ≥600 units/mg protein (biuret)	specific activity ≥400 units/mg protein (biuret)	specific activity 600-1000 units/mg protein (biuret)	specific activity 2000-4000 units/mg protein (modified Warburg-Christian)
form ammonium sulfate suspension	form ammonium sulfate suspension	form buffered aqueous glycerol solution	form ammonium sulfate suspension
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
Quality Level 200	Quality Level -	Quality Level 200	Quality Level 200
foreign activity Glutamic-Oxalacetic Transaminase ≤0.01%, Glutamic-Pyruvic Transaminase ≤0.01%	foreign activity Glutamic-Oxalacetic Transaminase ≤0.01%, Glutamic-Pyruvic Transaminase ≤0.01%	foreign activity Glutamic-Oxalacetic Transaminase ≤0.01%, Glutamic-Pyruvic Transaminase ≤0.01%	foreign activity -