M1421

IgA, Kappa from murine myeloma

clone TEPC 15, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Mouse IgA-κ
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00164795
• Conjugate: unconjugated
• Clone: TEPC 15, monoclonal
• Application: —
• Citations: 37

Properties

• biological source: mouse
• Quality Level: 300
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• clone: TEPC 15, monoclonal
• assay: ≥90% (non-reduced, SDS-PAGE)
• form: buffered aqueous solution
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

IgA antibody is secretory antibody and is present abundantly in mucous linings of gastrointestinal, respiratory and genitourinary tracts, tears and saliva. Thus secretory IgA contributes to the humoral defense mechanism against the pathogens on mucosal surfaces[1]
IgA, κ from murine myeloma is produced by TEPC 15 tumor line that is pristane-induced plasmacytoma originated and carried intraperitoneally in BALB/c mice. The antibody is specific for κ chain of mouse IgA. The purified immunoglobulin preparation is non-reactive with anti-mouse IgM, IgG1, IgG2a, IgG2b, and IgG3.

The TEPC 15 tumor line that produces mouse IgA,k is pristane-induced plasmacytoma originated and carried intraperitoneally in BALB/c mice. Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. IgA molecules in the serum are mostly dimeric. IgA is found in saliva, tracheobronchial secretions, genitourinary secretions, milk and colostrum.

Application

Anti-Mouse IgA, κ antibody was used as secondary antibody in ELISA[2][3][4][5] and immunoflourescence.[6]

IgA, Kappa from murine myeloma has been used in:

• immunofluorescence assay
• enzyme-linked immunosorbent assay (ELISA)
• invasion assays
• western blotting[7]

Biochem/physiol Actions

Antigen Specificity: phosphoryl choline

Immunoglobulin G (IgG) helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Physical form

Solution in 0.02 M Tris buffered saline, pH 8.0, containing 0.02% sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)