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M1302

Sigma-Aldrich

M-MLV Reverse Transcriptase

Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis

Synonym(s):

Moloney Murine Leukemia Virus Reverse Transcriptase

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.55

biological source

Porcine intestinal mucosa

Quality Level

recombinant

expressed in E. coli

form

liquid

usage

sufficient for 200 reactions
sufficient for 250 reactions

feature

dNTPs included: no
hotstart: no

concentration

200 units/μL

technique(s)

RT-PCR: suitable

color

colorless

input

purified RNA

shipped in

wet ice

storage temp.

−20°C

General description

Moloney murine leukemia virus (M-MLV ) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues. It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.

Application

M-MLV Reverse Transcriptase has been used:
  • for the preparation of cDNA libraries or for first strand cDNA synthesis
  • for use in a 2-step RT-PCR assay
  • in quantitative realtime-polymerase chain reaction (RT-qPCR)
  • in reverse transcription

Features and Benefits

  • Thermostable reverse transcriptase active at 37 °C.
  • Can be used to generate first strand cDNA of up to 7 kb.

Packaging

Supplied with 10× M-MLV reverse transcriptase buffer containing DTT.

Unit Definition

One unit incorporates 1 nmol of TTP into acid precipitable material in 10 min. at 37 °C using poly(A):oligo dT as a template:primer.

Preparation Note

The enzyme is purified from Escherichia coli expressing the pol gene of M-MLV on a plasmid.

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

inhibitor

Product No.
Description
Pricing

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Functional analysis of LHCSR1, a protein catalyzing NPQ in mosses, by heterologous expression in Arabidopsis thaliana
Dikaios I, et al.
Photosynthesis Research, 142, 249-264 (2019)
Downregulation of the PHLDA1 gene in IMR-32 neuroblastoma cells increases levels of Aurora A, TRKB and affects proteins involved in apoptosis and autophagy pathways
Durbas M, et al.
International Journal of Oncology, 49(2), 823-837 (2016)
Frost hardiness expression and characterisation in wheat at ear emergence
Al IM, et al.
Journal of agronomy and crop science, 199, 66-74 (2013)
Murine leukemia virus reverse transcriptase: structural comparison with HIV-1 reverse transcriptase
Cote ML and Roth MJ
Virus Research, 134, 186-202 (2008)
Downregulation of the PHLDA1 gene in IMR-32 neuroblastoma cells increases levels of Aurora A, TRKB and affects proteins involved in apoptosis and autophagy pathways
Durbas M, et al.
International Journal of Oncology, 49, 823-837 (2016)

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