L7395
Lipoxidase from Glycine max (soybean)
Type I-B, lyophilized powder, ≥50,000 units/mg solid
Synonym(s):
15-lipoxygenase, LOX A, Linoleate:oxygen oxidoreductase, Lipoxygenase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-853-1
MDL number:
EC Number:
Specific activity:
≥50,000 units/mg solid
Pricing and availability is not currently available.
type
Type I-B
Quality Segment
form
lyophilized powder
specific activity
≥50,000 units/mg solid
mol wt
94 kDa
shipped in
wet ice
storage temp.
−20°C
General description
Lipoxidases (LOXs), or lipoxygenases, are nonheme iron proteins. Lipoxygenases are widely present in plants, some microorganisms, and animals. They are categorized as LOX-1, LOX-2, and LOX-3 with respect to pH and optimum temperature, substrate specificity, and level of inhibition by different agents.
Application
Lipoxidase from Glycine max (soybean) has been used:
- to study the effects of 2-(α-D-glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol(TMG) on oxidation of lipoproteins[1]
- as a representative enzyme for lipoxygenases in microplate assay platform to screen for enzymes degrading wood extractives
- as a positive control in lipoxygenase inhibitory activity assay[2]
The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).
Biochem/physiol Actions
Lipoxidases are deactivated by heat treatment near 100°C for 5 to 10 minutes. Studies show that gamma irradiation treatment decreases the specific activity of soybean lipoxygenases. These enzymes are used in studies related to asthma and allergies. Lipoxidases are applicable for industrial bio-transformations such as in the production of aroma fine chemicals.
Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.
Physical form
Contains stabilizer and NaCl
Other Notes
One unit will cause an increase in A234 of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A234 unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥50,000 units/mg solid | specific activity 500,000-1,000,000 units/mg protein | specific activity - | specific activity - |
| form lyophilized powder | form ammonium sulfate suspension | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| mol wt 94 kDa | mol wt 94 kDa | mol wt - | mol wt - |
| shipped in wet ice | shipped in - | shipped in - | shipped in - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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