L6533

Lectin from Dolichos biflorus (horse gram)

biotin conjugate, buffered aqueous solution

• Synonym(s): Dolichos biflorus agglutinin, DBA
• NACRES: NA.32
• UNSPSC Code: 12352202

Properties

• conjugate: biotin conjugate
• Quality Segment: 200
• form: buffered aqueous solution
• composition: Protein, ≥2.5 mg/mL biuret
• extent of labeling: ≥3 mol biotin per mol lectin
• storage temp.: −20°C

Description

General description

Lectin from Dolichos biflorus (horse gram) is a heterotetrameric glycoprotein with a molecular mass of around 113 kDa. Dolichos biflorus agglutinin (DBA) is specific for terminal α-linked N-acetyl-D-galactosamine (GalNAc). It is a dietary lectin.

Application

Lectin from Dolichos biflorus (horse gram) has been used:

• to characterize mouse uterine natural killer (uNK) cells
• in double staining with lectins to localize nuclear factor-κB (NF-κB) activation
• as a species-specific gonocyte marker for the determination of donor cell colonization
• to label undifferentiated spermatogonia/cat testicular tissue

Biochem/physiol Actions

Dolichos biflorus agglutinin (DBA) possesses binding activity for which it is used as a marker binucleate cells and can indicate the state of their differentiation. It serves as a label for primordial germ cells (PGCs), gonocytes, pre-spermatogonia and spermatogonia.

DBA has anti-A₁ human blood group specificity, useful for distinguishing between A₁ and A₂ blood types. DBA has strong anti-Cad activity, and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.

Packaging

Sold on the basis of mg active conjugate.

Physical form

contains 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca²⁺

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable