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L4524

Lipopolysaccharides from Escherichia coli O55:B5

purified by ion-exchange chromatography, TLR ligand tested

Synonym(s):

LPS

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About This Item

CAS Number:
93572-42-0
UNSPSC Code:
12352201
EC Number:
297-473-0
NACRES:
NA.25
MDL number:
MFCD00164401
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biological source

Escherichia coli (O55:B5)

Quality Level

200

form

lyophilized powder

purified by

ion-exchange chromatography

impurities

<1% Protein, <1% RNA

color

white to yellow cast

solubility

water: soluble

shipped in

ambient

storage temp.

2-8°C

General description

This product is extracted from E. coli serotype O55:B5 and purified by ion exchange. The source strain is CDC 1644-70. The LPS O55:B5 has been used to stimulate human peritoneal macrophages at 1 ng/mL and to stimulate equine peritoneal macrophages at 1-100 ng/mL.

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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Lipopolysaccharides from Escherichia coli O55:B5 purified by ion-exchange chromatography, TLR ligand tested

Sigma-Aldrich

L4524

Lipopolysaccharides from Escherichia coli O55:B5

Lipopolysaccharides from Escherichia coli O127:B8 purified by ion-exchange chromatography, TLR ligand tested

Sigma-Aldrich

L5024

Lipopolysaccharides from Escherichia coli O127:B8

Lipopolysaccharides from Escherichia coli O111:B4 purified by ion-exchange chromatography, TLR ligand tested

Sigma-Aldrich

L3024

Lipopolysaccharides from Escherichia coli O111:B4

Lipopolysaccharides from Escherichia coli O55:B5 purified by trichloroacetic acid extraction

Sigma-Aldrich

L4005

Lipopolysaccharides from Escherichia coli O55:B5

biological source

Escherichia coli (O55:B5)

biological source

Escherichia coli (O127:B8)

biological source

Escherichia coli (O111:B4)

biological source

Escherichia coli (O55:B5)

solubility

water: soluble

solubility

water: soluble

solubility

water: soluble

solubility

water: soluble

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

impurities

<1% Protein, <1% RNA

impurities

<1% Protein, <1% RNA

impurities

<1% Protein, <1% RNA

impurities

1-10% Protein (Lowry)

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pictograms

Skull and crossbones
GHS06

signalword

Danger

hcodes

H300

Hazard Classifications

Acute Tox. 2 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000476847
0000476847
0000476849
0000476848
0000476848

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Arij Faksh et al.
Pediatric research, 79(3), 391-400 (2015-11-06)
Antenatal inflammation and preterm birth are associated with the development of airway diseases such as wheezing and asthma. Utilizing a newborn mouse model, we assessed the effects of maternal inflammation and postnatal hyperoxia on the neonatal airway. Pregnant C57/Bl6 dams
Hung-Yen Chou et al.
PloS one, 13(5), e0196890-e0196890 (2018-05-09)
The purple sea urchin, Strongylocentrotus purpuratus, has a complex and robust immune system that is mediated by a number of multi-gene families including the SpTransformer (SpTrf) gene family (formerly Sp185/333). In response to immune challenge from bacteria and various pathogen-associated
Laura B Fanning et al.
PloS one, 8(2), e57007-e57007 (2013-02-23)
Leukocyte Immunoglobulin-like Receptor B4 (LILRB4) null mice have an exacerbated T helper cell type 2 (Th2) immune response and pulmonary inflammation compared with Lilrb4(+/+) animals when sensitized intranasally with ovalbumin (OVA) and low-dose lipopolysaccharide (LPS) followed by challenge with OVA.
View All Related Papers

Global Trade Item Number

SKUGTIN
L4524-5MG04061833957592
L4524-10MG04061833272473
L4524-25MG04061833957585

Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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