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L4268

Lipopolysaccharides from Klebsiella pneumoniae

purified by phenol extraction

Lipopolysaccharides from Klebsiella pneumoniae

Synonym(s):

LPS

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About This Item

UNSPSC Code:
12352201
NACRES:
NA.25
MDL number:
MFCD00131520
Pricing and availability is not currently available.
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biological source

bacterial (Klebsiella pneumoniae)

Quality Segment

200

form

lyophilized powder

purified by

phenol extraction

impurities

<3% Protein (Lowry)

color

white to faint yellow

solubility

water: 4.90-5.10 mg/mL, slightly hazy to turbid, colorless to faintly yellow

shipped in

ambient

storage temp.

2-8°C

General description

This product is phenol extracted from Klebsiella pneumoniae. The source strain is ATCC 15380.

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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biological source

bacterial (Klebsiella pneumoniae)

biological source

Pseudomonas aeruginosa (10)

biological source

Escherichia coli (K-235)

biological source

bacterial (Salmonella typhosa)

solubility

water: 4.90-5.10 mg/mL, slightly hazy to turbid, colorless to faintly yellow

solubility

water: 4.90-5.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow

solubility

water: soluble

solubility

water: 4.90-5.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

impurities

<3% Protein (Lowry)

impurities

<3% Protein (Lowry)

impurities

<3% Protein (Lowry)

impurities

<3% Protein (Lowry)

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000419210
0000419210
0000419209
0000419209
0000410664

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Global Trade Item Number

SKUGTIN
L4268-10MG04061833272466
L4268-100MG04061833272459
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Questions

  1. Is the product LPS from K. pneumoniae Cat N L4268 Ultrapure? As prevention to minimisation effect of the mixture in cell culture

    1 answer

    1. This product is purified by phenol extraction and is not considered ‘Ultrapure’. Currently, the term ‘Ultrapure’ is not assigned to any of the LPS products. However, the method of purification is highly indicative of the type and amount of contaminants that may be present. Of the various methods of preparation and purification available, the most pure products are those purified by ion exchange chromatography followed by those purified by gel filtration. Unfortunately, a chromatography purified version of LPS from Klebsiella is not currently available.
      Please see the link below for more information on the results of this type of purification.
      https://www.sigmaaldrich.com/technical-documents/technical-article/research-and-disease-areas/cell-signaling/lipopolysaccharides

      Please see the link below to review LPS products sorted by the purification method:
      https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/research-and-disease-areas/immunology-research/lipopolysaccharides-for-research

      Please see the following link to review the general handling and usage information available for LSP products:
      https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides

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