L3137
Lipopolysaccharides from Escherichia coli O127:B8
purified by gel-filtration chromatography
Synonym(s):
LPS
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About This Item
Pricing and availability is not currently available.
biological source
Escherichia coli (O127:B8)
Quality Level
form
lyophilized powder
purified by
gel-filtration chromatography
impurities
<1% Protein
color
white to yellow cast
solubility
water: soluble
shipped in
ambient
storage temp.
2-8°C
General description
This product is extracted from E. coli serotype O127:B8 and purified by gel filtration. The source strain is ATCC 12740. This LPS serotype has been used in the study of septic shock and to induce NOS in murine macrophages and PAF synthesis in rat glomerular masangial cells.
Application
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Biochem/physiol Actions
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
Preparation Note
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
Other Notes
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
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This Item | |||
|---|---|---|---|
| biological source Escherichia coli (O127:B8) | biological source Escherichia coli (O111:B4) | biological source Escherichia coli (O26:B6) | biological source Escherichia coli (O55:B5) |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| solubility water: soluble | solubility water: soluble | solubility water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow | solubility water: soluble |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| impurities <1% Protein | impurities <1% Protein | impurities <5% Protein | impurities <3% Protein |
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signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 2 Oral
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Eugene S Yim et al.
Journal of biomedical materials research. Part A, 91(3), 894-902 (2008-12-17)
Hydrogel polymers comprise a novel category of synthetic materials being investigated for use in cartilage replacement. One candidate compound, a poly(ethylene glycol)/poly(acrylic acid) (PEG/PAA) interpenetrating polymer network (IPN), was developed for use in corneal prostheses and was recently engineered for
Evelin Painsipp et al.
PloS one, 6(6), e20719-e20719 (2011-06-16)
Immune challenge by bacterial lipopolysaccharide (LPS) causes short-term behavioral changes indicative of depression. The present study sought to explore whether LPS is able to induce long-term changes in depression-related behavior and whether such an effect depends on mouse strain and
Choon Kit Tang et al.
Biomaterials, 30(7), 1389-1400 (2008-12-09)
Receptor mediated gene delivery is an attractive non-viral method for targeting genetic material to specific cell types. We have previously utilized oxidized (OMPLL) and reduced mannan poly-L-lysine (RMPLL) to target DNA vaccines to antigen presenting cells and demonstrated that it
Global Trade Item Number
| SKU | GTIN |
|---|---|
| L3137-10MG | 04061833272275 |
| L3137-25MG | 04061833272282 |
| L3137-5MG | 04061833950845 |