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IMDM

Name: IMDM
Brand: SIGMA

With sodium bicarbonate and ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Iscove′s MDM, Iscoves MDM, Iscove’s Modified Dulbecco’s Medium, IMDM

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217469

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Properties

Product Name

Iscove′s Modified Dulbecco′s Medium, With sodium bicarbonate and L-glutamine, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: yes
phenol red: yes
NaHCO₃: yes
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

Description

General description

Iscove′s Modified Dulbecco′s Medium contains selenium, additional amino acids and vitamins, sodium pyruvate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and potassium nitrate instead of ferric nitrate, and is a modification of Dulbecco′s Modified Eagle′s medium (DME). Iscove′s medium can be used to support murine B lymphocytes, bone marrow hemopoietic tissue, B cells stimulated with lipopolysaccharide, T lymphocytes, and various hybrid cells.

Application

Iscove′s Modified Dulbecco′s Medium has been used to culture:

human hepatocellular carcinoma (HCC) cell line HepG2 as monolayers[1]
to culture synovial tissue samples[2]
bacterial strain inoculum for stimulation of immune cell experiments[3]

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This Item	I7633	I3390	51471C
SAFC I6529 Iscove′s Modified Dulbecco′s Medium	Sigma-Aldrich I7633 Iscove′s Modified Dulbecco′s Medium	Sigma-Aldrich I3390 Iscove′s Modified Dulbecco′s Medium	Sigma-Aldrich 51471C Iscove′s Modified Dulbecco′s Medium
technique(s) cell culture \| hybridoma: suitable, cell culture \| mammalian: suitable	technique(s) cell culture \| hybridoma: suitable, cell culture \| mammalian: suitable	technique(s) cell culture \| hybridoma: suitable, cell culture \| mammalian: suitable	technique(s) cell culture \| hybridoma: suitable, cell culture \| mammalian: suitable, cell culture \| stem cell: suitable
sterility sterile-filtered	sterility -	sterility sterile-filtered	sterility sterile
form liquid	form powder	form liquid	form liquid
components glucose: yes phenol red: yes NaHCO₃: yes L-glutamine: yes	components phenol red: yes glucose: yes L-glutamine: yes	components phenol red: yes L-glutamine: no glucose: yes	components phenol red: 15.93 mg/L NaHCO₃: 3024 mg/L L-glutamine: 582 mg/L HEPES: 5958 mg/L sodium pyruvate: 110 mg/L
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Global Trade Item Number

SKU	GTIN
I6529-500ML	04061837847240

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