I2778

IL-1α human

≥98% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): IL-1α, hIL-1α., Interleukin-1α human, Lymphocyte activating factor
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00164814
• Biological source: human
• Recombinant: expressed in E. coli
• Assay: ≥98% (SDS-PAGE and HPLC)
• Form: lyophilized powder
• Mol wt: ~17 kDa

Properties

• Product Name: Interleukin-1α human, IL-1α, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• biological source: human
• Quality Segment: 100
• recombinant: expressed in E. coli
• assay: ≥98% (SDS-PAGE and HPLC)
• form: lyophilized powder
• quality: endotoxin tested
• mol wt: ~17 kDa
• packaging: pkg of 2 and 10 μg
• technique(s): cell culture | mammalian: suitable
• UniProt accession no.: P01583
• storage temp.: −20°C
• Gene Information: human ... IL1A(3552)

Description

General description

Interleukin-1α (IL-1α) is a cytokine that belongs to the interleukin-1 (IL-1) family.[1] It is located intracellularly in hematopoietic and non-hematopoietic cells.[2] IL-1α gene is located on the human chromosome at 2q14.1.

Application

Interleukin-1α (IL-1α) has been used:

• to study its effects on human colorectal adenocarcinoma cells[3]
• to stimulate primary human fetal retinal pigment epithelium (hfRPE) cells[4]
• to pre-stimulate human THP-1 monocytes[5]

Biochem/physiol Actions

Interleukin-1α (IL-1α) plays a crucial role in inflammation during cell necrosis. It also regulates the recruitment of interleukin-1β (IL-1β), neutrophils, chemokines, and other cytokines at the site of injury. IL-1α exhibits a dual function of binding to its cell surface receptor as well as chromatin.[1]

Physical form

Lyophilized from 0.2 μm filtered 10 mM sodium phosphate , pH 8.0, plus 50 mm NaCl and 50 μg bovine serum albumin (BSA) per μg cytokine.

Analysis Note

The proliferative activity of human IL-1α is tested in culture using the murine cell line D10S.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319,H335
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• ppe: dust mask type N95 (US), Eyeshields, Gloves
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• flash_point_f: No data available
• flash_point_c: No data available