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Isocitric Dehydrogenase (NADP) from porcine heart

Name: Isocitric Dehydrogenase (NADP) from porcine heart
Brand: SIGMA

Type I, 0.5-3.0 unit/mg solid (plus numerous enzyme activities associated with porcine heart)

Synonym(s):

threo-D_s-Isocitrate: NADP⁺ oxidoreductase, ICDH, IDH

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About This Item

CAS Number:

9028-48-2

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-835-3

MDL number:

MFCD00131420

EC Number:

1.1.1.42 (BRENDA, IUBMB)

Specific activity:

0.5-3.0 unit/mg solid (plus numerous enzyme activities associated with porcine heart)

Biological source:

Porcine heart

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Properties

biological source

Porcine heart

Quality Segment

200

type

Type I

form

solid

specific activity

0.5-3.0 unit/mg solid (plus numerous enzyme activities associated with porcine heart)

storage temp.

−20°C

Description

Biochem/physiol Actions

Isocitrate dehydrogenase (IDH) plays a major role in the Krebs cycle by catalyzing the oxidative decarboxylation of isocitrate. The oxidative decarboxylation of isocitrate generates α-ketoglutarate and NADPH. NADPH produced is essential for the production of fatty acids and cholesterol. This NADP-dependent molecules serves as homodimers.

Analysis Note

Crude

Other Notes

One unit will convert 1.0 μmole of isocitrate to α-ketoglutarate per min at pH 7.4 at 37°C.

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This Item	I2002	L7525	A5384
Sigma-Aldrich I1877 Isocitric Dehydrogenase (NADP) from porcine heart	Sigma-Aldrich I2002 Isocitric Dehydrogenase (NADP) from porcine heart	Sigma-Aldrich L7525 L-Lactic Dehydrogenase from porcine heart	Sigma-Aldrich A5384 Aconitase from porcine heart
biological source Porcine heart	biological source -	biological source Porcine heart	biological source Porcine heart
specific activity 0.5-3.0 unit/mg solid (plus numerous enzyme activities associated with porcine heart)	specific activity 3-20 units/mg protein	specific activity ≥200 units/mg protein	specific activity -
form solid	form buffered aqueous glycerol solution	form ammonium sulfate suspension	form solid
storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
type Type I	type Type IV	type -	type -