We do not recommend re-using the columns. Each column is intended for one time use.
H7787
HIS-Select® Spin Columns
Synonym(s):
His-Tag Spin Columns
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About This Item
NACRES:
NA.56
UNSPSC Code:
41106500
Pore size:
100 nm pore size
Packaging:
pkg of 10 ea, pkg of 50 ea
Pricing and availability is not currently available.
shelf life
2 yr at 2‑8 °C
Quality Segment
packaging
pkg of 10 ea, pkg of 50 ea
pore size
100 nm pore size
capacity
>0.5 mg/column
storage temp.
2-8°C
General description
Kit contains 3 collection tubes for every spin column.
Application
Pre-packed and ready-to-use HIS-Select Spin Columns allow for fast and consistent purification of small-scale crude cell extracts containing histidine-tagged proteins. The spin columns contain a matrix of silica particles and uses the HIS-Select patented non-charged, hydrophilic nickel chelating linkage chemistry. HIS-Select Spin Columns are highly selective for histidine-tagged proteins and exhibit very low non-specific binding of other proteins. These convenient Spin Columns allow for fast, one-step purification for the isolation of at least 150 μg of histidine-tagged protein in less than 15 minutes.
Features and Benefits
- High Selectivity for higher purity
- Convenient and Ready-to-Use
- Consistent protein binding each use
- Compatible with CelLytic™ cell lysis reagents
Legal Information
CelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| pore size 100 nm pore size | pore size - | pore size - | pore size 3-5 μm |
| packaging pkg of 10 ea, pkg of 50 ea | packaging pkg of 1 kit | packaging - | packaging - |
| storage temp. 2-8°C | storage temp. 20-25°C | storage temp. room temp | storage temp. - |
| Quality Level 200 | Quality Level - | Quality Level 200 | Quality Level - |
| capacity >0.5 mg/column | capacity - | capacity - | capacity 4 mg binding capacity |
| shelf life 2 yr at 2‑8 °C | shelf life - | shelf life - | shelf life - |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Maria A Schumacher et al.
Nucleic acids research, 46(14), 7405-7417 (2018-06-16)
Streptomyces are filamentous bacteria with a complex developmental life cycle characterized by the formation of spore-forming aerial hyphae. Transcription of the chaplin and rodlin genes, which are essential for aerial hyphae production, is directed by the extracytoplasmic function (ECF) σ
Tian Luo et al.
Clinical and vaccine immunology : CVI, 17(1), 87-97 (2009-12-04)
Recently, major species-specific antibody epitopes in three immunoreactive tandem repeat proteins (TRPs) of Ehrlichia chaffeensis, TRP32, TRP47, and TRP120, have been identified and molecularly characterized within tandem repeat (TR) regions. In this study, we mapped the major immunodeterminants of the
Michael D BeGora et al.
The Journal of biological chemistry, 285(38), 29147-29155 (2010-07-24)
Three sequential methylations of phosphoethanolamine (PEA) are required for the synthesis of phosphocholine (PCho) in plants. A cDNA encoding an N-methyltransferase that catalyzes the last two methylation steps was cloned from Arabidopsis by heterologous complementation of a Saccharomyces cerevisiae cho2
Global Trade Item Number
| SKU | GTIN |
|---|---|
| H7787-10EA | 04061835573271 |
| H7787-50EA | 04061833804759 |
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Can HIS-Select® Spin Column, Product H7787, be re-used?
1 answer-
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What is the binding capacity of the HIS-Select® Spin Column, Product H7787?
1 answer-
The binding capacity of a HIS-Select Spin Column is >500 ug per column as determined witha ~30 kDa histidine tagged protein recovered under high levels of protein expression.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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