H6136
Hanks′ Balanced Salts
Without sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
Hanks′ Balanced Salt Solution
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About This Item
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General description
Balanced Salt solutions (BSS) are essential in cell culture as they serve as irrigating, transporting, and diluting agents to maintain the osmotic balance between intra-and extracellular spaces. They provide cells with water, inorganic ions, and energy for normal cell metabolism. BSS also provides a buffering system to maintain the medium within the optimum pH range (7.2-7.6).
Application
Hanks′ Balanced Salt has been used:
- in the preparation of stock solution for culturing mice primary hepatocytes[1]
- to measure the mineralized nodule formation in human osteoblast cultures
- in the preparation of embryo buffer to collect zebrafish eggs
Preparation Note
Formulated to contain 9.8 grams of powder per liter of medium.
Supplement with 0.35 g/L sodium bicarbonate.
Other Notes
The pH is adjusted after addition of sodium bicarbonate.
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This Item | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 500 | Quality Level 500 | Quality Level - | Quality Level 500 |
| form powder | form powder | form powder | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
HBSS, salt solutions | HBSS, salt solutions | HBSS, salt solutions | HBSS, salt solutions |
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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