H5416

FLT3 Ligand human

HumanKine^®, recombinant, expressed in HEK 293 cells, suitable for cell culture

• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD01634859
• Biological source: human
• Recombinant: expressed in HEK 293 cells
• Assay: ≥95% (SDS-PAGE)
• Form: lyophilized powder
• Mol wt: monomer 24-30 kDa (glycosylated)
• Impurities: ≤1 EU/mg

Properties

• Product Name: FLT3 Ligand human, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture
• biological source: human
• Quality Segment: 100
• recombinant: expressed in HEK 293 cells
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤0.8 ng/mL EC₅₀
• quality: endotoxin tested
• mol wt: monomer 24-30 kDa (glycosylated)
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/mg
• storage temp.: −20°C

Description

General description

Human FMS-like tyrosine kinase 3 (FLT3) ligand is a homodimer consisting of two short-chain α-helical bundles. The molecular weight of FLT3 ligand is 44 kDa.

Biochem/physiol Actions

FLT3 ligand binds to the cell-bound tyrosine kinase receptor and activates them.

HumanKine FLT3 Ligand is expressed in human HEK 293 cells as a glycosylated monomer with an apparent molecular mass of 24-30 kDa. HumanKine FLT3 Ligand in human HEK 293 cells contributes to glycosylation.FLT3 ligand is a hematopoietic cytokine that regulates the proliferation of early hematopoietic cells. It has been shown to synergize with a variety of cytokines to stimulate the growth and differentiation of early hematopoietic cells. The FLT3 ligand also stimulates the expansion of monocytes and immature dendritic cells, and induces early B cell lineage differentiation and NK cell growth.

Physical form

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of the human acute myeloid leukemia cell line OCI-AML5.

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable