This product is prepared from bovine red blood cells collected from USDA approved abbatoirs. The cattle are typically intended for the food industry.
H2625
Hemoglobin from bovine blood
suitable for protease substrate, substrate powder
Synonym(s):
Bovine hemoglobin, Hb, Methemoglobin
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About This Item
Form:
substrate powder
Biological source:
bovine blood
Mol wt:
Mr ~64500
Pricing and availability is not currently available.
biological source
bovine blood
Quality Segment
form
substrate powder
mol wt
Mr ~64500
technique(s)
activity assay: suitable
solubility
H2O: soluble 20 mg/mL
suitability
suitable for protease substrate
storage temp.
2-8°C
Gene Information
General description
Hemoglobin is the major component of red blood cells and is responsible for their red color. Its normal concentration in erythrocytes is 34%. Hemoglobin is a globular protein with α and β chains with every 141 and 146 amino acids respectively. It exists as a tetramer with each monomer having heterocyclic porphyrin ring with iron constituting the heme.
Application
Hemoglobin from bovine blood has been used as a substrate in cathepsin D activity assay. It has also been used to measure the activity of acid proteases (pepsin-like) in the stomach extract.
The solubility of α-elastin has been applied to construction of elastin-mimetic biomaterials.
Biochem/physiol Actions
Bovine hemoglobin is used in the production of hemoglobin-vesicles (HbV). It has high thermal stability and high oxygen affinity when compared to human hemoglobin. Bovine hemoglobin interacts with synthetic and azo dyes. Polymerized forms of bovine hemoglobin are used to treat autoimmune hemolytic anemia.
Oxygen transporter, NO scavenger
Oxygen transporter. The Fe2+/Fe3+ balance is a physiological indicator of blood oxygenation; deoxygenated hemoglobin accessorizes a feedback loop by reducing nitrite to NO, a vasodilator which enhances blood flow to oxygen-deprived tissues.
Preparation Note
Prepared from washed, lysed and dialyzed erythrocytes.
Disclaimer
Since native hemoglobin is readily oxidized in air, these preparations may be predominantly methemoglobin.
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This Item | |||
|---|---|---|---|
| biological source bovine blood | biological source bovine blood | biological source bovine blood | biological source - |
| technique(s) activity assay: suitable | technique(s) - | technique(s) - | technique(s) - |
| mol wt Mr ~64500 | mol wt Mr ~64500 | mol wt Mr ~64500 | mol wt Mr ~64500 |
| form substrate powder | form powder | form dried erythrocytes | form solid |
| suitability suitable for protease substrate | suitability - | suitability - | suitability - |
| solubility H2O: soluble 20 mg/mL | solubility - | solubility 0.6 M HCl: soluble 20 mg/mL | solubility - |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Is this product a residue from the meat and food industry?
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If made up in solution and stored at 2-8C, how long can this product last for?
1 answer-
Unfortunately, the solution stability of this material at 2-8°C has not been determined. However, solutions may be stored in aliquots at -20°C for up to 3 months.
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Is this product suitable for mammalian cell culture?
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H2625 is specified to be suitable as a substrate in a protease assay. It is not evaluated for cell culture use.
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Do you have any information on purity of this product?
1 answer-
This product has not been tested for purity. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/400/152/h2500pis.pdfHelpful?
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