F8435

PNGase F Glycosidase

Removes N-linked glycans, suitable for mass spectrometry, recombinant, expressed in E. coli

• Synonym(s): N-Glycosidase F, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum, Peptide N-glycosidase
• CAS Number: 83534-39-8
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 3.5.1.52 (BRENDA, IUBMB)
• MDL number: MFCD18839214
• Specific activity: ≥1,000 U/mg
• Recombinant: expressed in E. coli
• Shelf life: ≥1 weeks at 2‑8 °C (for reconstituted solution), ≥1 yr at -20 °C

Properties

• Product Name: PNGase F from Elizabethkingia meningoseptica, lyophilized powder, recombinant, expressed in E. coli
• recombinant: expressed in E. coli
• Quality Level: 200
• conjugate: (N-linked)
• grade: Proteomics Grade
• form: lyophilized powder
• specific activity: ≥1,000 U/mg
• shelf life: ≥1 weeks at 2‑8 °C (for reconstituted solution), ≥1 yr at -20 °C
• mol wt: ~36 kDa
• shipped in: wet ice
• storage temp.: −20°C

Description

Application

Highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-teminal 6x histidine fusion tag.

Used to deglycosylate protein.

Biochem/physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

Packaging

PNGase F was used for deglycosylation of P-glycoprotein in a study to investigate the dual impact of statins on p-glycoprotein and its effect on doxorubicin cytotoxicity in human neuroblastoma cells.[1] It was used to treat a purified protein, mouse cone ultraviolet (MUV) pigment, before use in quantitative immunoblot analysis in a study[2] It is used to deglycosylate N-linked glycoproteins. Highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-teminal 6x histidine fusion tag.

Other Notes

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable