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EDTA Buffer, pH 8.5, 10×, Antigen Retriever

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About This Item

NACRES:

NA.47

UNSPSC Code:

12352107

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Properties

pH

8.5

Quality Segment

200

application(s)

hematology
histology

storage temp.

2-8°C

Description

Application

Used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. The use of EDTA buffer, pH 8.5, or other antigen retrieval solutions on FFPE tissue sections improves accessibility of antibodies to tissue antigens. EDTA buffer has been used to heat tissue sections in order to visualize antigens by immunohistochemical analysis.

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This Item	C9999	T6455	2851
Sigma-Aldrich E1161 EDTA Buffer, pH 8.5, 10×, Antigen Retriever	Sigma-Aldrich C9999 Citrate Buffer, pH 6.0, 10×, Antigen Retriever	Sigma-Aldrich T6455 Tris-HCl Buffer, pH 10, 10×, Antigen Retriever	Sigma-Aldrich 2851 Citrate Phosphate Buffer Concentrate
Quality Level 200	Quality Level 100	Quality Level 100	Quality Level 500
application(s) hematology histology	application(s) hematology histology	application(s) hematology histology	application(s) hematology histology
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
pH 8.5	pH 6.0	pH 10	pH -